PMID- 9639883
OWN - NLM
STAT- MEDLINE
DCOM- 19980710
LR  - 20190720
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 806
IP  - 1
DP  - 1998 May 8
TI  - Analysis of clinically relevant, diagnostic DNA by capillary zone and 
      double-gradient gel slab electrophoresis.
PG  - 97-112
AB  - A number of applications of capillary zone electrophoresis (CZE) in sieving 
      liquid polymers (notably linear polyacrylamides and cellulose) for the analysis 
      of polymerase chain reaction products of clinically relevant, diagnostic DNA, are 
      reviewed here. The fields covered are human genetics, quantitative gene dosage, 
      microbiology and virology, forensic medicine and therapeutic DNA (notably 
      antisense nucleotides). Some unique, novel developments are highlighted, such as 
      (a) non-isocratic CZE, i.e., temperature-programmed CZE for detection of DNA 
      point mutations and (b) the synthesis of novel N-substituted acrylamides, 
      offering extreme resistance to alkaline hydrolysis, coupled with high 
      hydrophilicity. In the field of denaturing gradient gel electrophoresis (DGGE), 
      as routinely performed in gel slabs, a novel methodology is described, i.e., 
      double-gradient DGGE. In this technique, two gradients are simultaneously applied 
      along the migration direction; a chemical denaturing gradient, for partially 
      unwinding homo- and hetero-duplexes of DNA and a porosity gradient, for 
      re-compacting diffuse bands melting over a broader range of denaturing 
      conditions. Both the CZE and the slab gel methodologies, with the latest 
      developments described in this review, appear to be promising tools for screening 
      diagnostic DNA.
FAU - Righetti, P G
AU  - Righetti PG
AD  - University of Verona, Department of Agricultural and Industrial Biotechnologies, 
      Italy.
FAU - Gelfi, C
AU  - Gelfi C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Viral)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/*analysis
MH  - DNA, Bacterial/analysis
MH  - DNA, Viral/analysis
MH  - Electrophoresis, Capillary/*methods
MH  - Forensic Medicine
MH  - Gene Dosage
MH  - Genetic Diseases, Inborn/*diagnosis/*genetics
MH  - Humans
RF  - 94
EDAT- 1998/06/26 00:00
MHDA- 1998/06/26 00:01
CRDT- 1998/06/26 00:00
PHST- 1998/06/26 00:00 [pubmed]
PHST- 1998/06/26 00:01 [medline]
PHST- 1998/06/26 00:00 [entrez]
AID - S0021-9673(97)00304-X [pii]
AID - 10.1016/s0021-9673(97)00304-x [doi]
PST - ppublish
SO  - J Chromatogr A. 1998 May 8;806(1):97-112. doi: 10.1016/s0021-9673(97)00304-x.