PMID- 964025
OWN - NLM
STAT- MEDLINE
DCOM- 19761121
LR  - 20190727
IS  - 0040-8727 (Print)
IS  - 0040-8727 (Linking)
VI  - 118 Suppl
DP  - 1976
TI  - Erythrocyte rosette forming activity of macrophage.
PG  - 199-205
AB  - Erythrocyte rosette forming activities of the rat lung macrophage, peritoneal 
      macrophage, Kupffer cells and expressed splenic cells were studied. The 
      erythrocytes employed were fresh human red blood cell (RBC), fresh sheep RBC 
      (SRBC), and tanned SRBC coated with bovine albumin or with bovine gamma-globulin. 
      The macrophages were mixed with RBC at a ratio of 1 to 100 or 200 in gelatin 
      veronal buffer or phosphate buffered saline. The experiments were carried out at 
      4 degrees C. No autologous serum nor anti-RBC serum was added to the experimental 
      system. 10 to 20 per cent of the lung macrophages formed rosette and their entire 
      surfaces were covered with RBC. The peritoneal macrophage and Kupffer cells were 
      also capable of forming rosette with fresh SRBC as well as with SRBC coated with 
      bovine albumin or with bovine gamma-globulin. Examinations for cell surface 
      immunoglobulin of alveolar macrophage, peritoneal macrophage, Kupffer cells and 
      splenic cells revealed that alveolar and peritoneal macrophages apparently 
      possessed surface gamma-globulin and that almost all of these macrophages 
      possessed surface immuno-globulin. In order to examine the effect of different 
      natures of SRBC on in vivo phagocytosis, rats were immunized with SRBC by 
      injecting various SRBC into the femoral or the portal vein and the hemagglutinin 
      titer was studied. The maximum hemagglutinin titer was observed in the rat 
      injected with fresh SRBC into the femoral vein and the minimum hemagglutinin 
      titer was observed in the splenectomized rat injected with fresh SRBC and in the 
      rat injected with SRBC coated with albumin or gamma-globulin into the femoral or 
      portal vein. Immunological role of Kupffer cells was discussed.
FAU - Ouchi, E
AU  - Ouchi E
FAU - Seiji, K
AU  - Seiji K
FAU - Sato, J
AU  - Sato J
FAU - Nomura, N
AU  - Nomura N
FAU - Watabe, S
AU  - Watabe S
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Tohoku J Exp Med
JT  - The Tohoku journal of experimental medicine
JID - 0417355
RN  - 0 (Antibodies)
SB  - IM
MH  - Animals
MH  - Antibodies
MH  - Ascitic Fluid/cytology
MH  - Cell Membrane/*immunology
MH  - Erythrocytes/*immunology
MH  - Humans
MH  - *Immune Adherence Reaction
MH  - Kupffer Cells/*immunology
MH  - Liver/immunology
MH  - Lung/cytology
MH  - Macrophages/*immunology
MH  - Male
MH  - Phagocytosis
MH  - Rats
MH  - Spleen/cytology
EDAT- 1976/01/01 00:00
MHDA- 1976/01/01 00:01
CRDT- 1976/01/01 00:00
PHST- 1976/01/01 00:00 [pubmed]
PHST- 1976/01/01 00:01 [medline]
PHST- 1976/01/01 00:00 [entrez]
AID - 10.1620/tjem.118.suppl_199 [doi]
PST - ppublish
SO  - Tohoku J Exp Med. 1976;118 Suppl:199-205. doi: 10.1620/tjem.118.suppl_199.