PMID- 9644205
OWN - NLM
STAT- MEDLINE
DCOM- 19980814
LR  - 20220110
IS  - 0172-8083 (Print)
IS  - 0172-8083 (Linking)
VI  - 33
IP  - 6
DP  - 1998 Jun
TI  - Editing status of mat-r transcripts in mitochondria from two plant species: 
      C-to-U changes occur in putative functional RT and maturase domains.
PG  - 420-8
AB  - The intronic mat-r ORF encodes a protein with significant homology to retroviral 
      reverse transcriptases. Here, we describe the nucleotide sequence of potato mat-r 
      and study the editing status of mat-r transcripts in two systems, potato and 
      wheat, where the mat-r ORF is part of the trans-introns but in two different 
      configurations relative to nad1 exons d and e. In potato and wheat, 13 and 15 
      C-to-U transitions respectively were observed. Most transcripts were partially 
      edited, but potato transcripts were edited more efficiently than wheat 
      transcripts. As in functional mitochondrial genes, RNA editing increased the 
      similarity between plant mat-r proteins and their homologous non-plant 
      counterparts. Interestingly, editing of mat-r was clustered in the 
      reverse-transcriptase (RT) and the maturase (X) domains, two well defined regions 
      having known functions in other systems. These results, together with the 
      integrity and sequence conservation of mat-r, strongly suggest that the encoded 
      protein plays a functional role in plant mitochondria.
FAU - Begu, D
AU  - Begu D
AD  - Laboratoire de Replication et Expression des Genes Eucaryotes et Retroviraux, 
      EP-630, C.N.R.S.-Universite de Bordeaux II, 1, rue Camille Saint-Saens, F-33077 
      Bordeaux Cedex, France.
FAU - Mercado, A
AU  - Mercado A
FAU - Farre, J C
AU  - Farre JC
FAU - Moenne, A
AU  - Moenne A
FAU - Holuigue, L
AU  - Holuigue L
FAU - Araya, A
AU  - Araya A
FAU - Jordana, X
AU  - Jordana X
LA  - eng
SI  - GENBANK/AJ003130
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Curr Genet
JT  - Current genetics
JID - 8004904
RN  - 0 (DNA, Mitochondrial)
RN  - 0 (DNA, Plant)
RN  - 0 (MAT-R protein, plant)
RN  - 0 (Plant Proteins)
RN  - 0 (RNA, Mitochondrial)
RN  - 56HH86ZVCT (Uracil)
RN  - 5CSZ8459RP (Cytidine)
RN  - 63231-63-0 (RNA)
RN  - 8J337D1HZY (Cytosine)
RN  - EC 2.7.7.- (Nucleotidyltransferases)
RN  - EC 2.7.7.- (mRNA maturase)
RN  - EC 2.7.7.49 (RNA-Directed DNA Polymerase)
RN  - EC 3.1.- (Endoribonucleases)
RN  - WHI7HQ7H85 (Uridine)
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Binding Sites/genetics/physiology
MH  - Cytidine/genetics/metabolism
MH  - Cytosine/metabolism
MH  - DNA, Mitochondrial/analysis/genetics
MH  - DNA, Plant/analysis/genetics
MH  - Endoribonucleases/*genetics/isolation & purification
MH  - Genes, Plant/genetics
MH  - Mitochondria/chemistry/*genetics
MH  - Molecular Sequence Data
MH  - Nucleotidyltransferases/*genetics/isolation & purification
MH  - Plant Proteins/*genetics
MH  - RNA/genetics/metabolism
MH  - RNA Editing/*genetics
MH  - RNA, Mitochondrial
MH  - RNA-Directed DNA Polymerase/genetics
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Solanum tuberosum/chemistry/*genetics
MH  - Transcription, Genetic/genetics
MH  - Triticum/chemistry/*genetics
MH  - Uracil/metabolism
MH  - Uridine/genetics/metabolism
EDAT- 1998/06/30 00:00
MHDA- 1998/06/30 00:01
CRDT- 1998/06/30 00:00
PHST- 1998/06/30 00:00 [pubmed]
PHST- 1998/06/30 00:01 [medline]
PHST- 1998/06/30 00:00 [entrez]
AID - 10.1007/s002940050355 [doi]
PST - ppublish
SO  - Curr Genet. 1998 Jun;33(6):420-8. doi: 10.1007/s002940050355.