PMID- 9646462
OWN - NLM
STAT- MEDLINE
DCOM- 19980724
LR  - 20220316
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 61
IP  - 1-2
DP  - 1998 Mar 15
TI  - Experimental inoculation of swine at various stages of gestation with a Danish 
      isolate of porcine reproductive and respiratory syndrome virus (PRRSV).
PG  - 21-31
AB  - Following intranasal inoculation of three groups of pregnant swine (in total 11 
      dams) with a Danish isolate of porcine reproductive and respiratory syndrome 
      virus (PRRSV) on or about day 85, 70 and 45 of gestation, respectively, 
      reproductive disturbances were observed in the first two groups. Transplacental 
      transmission of PRRSV occurred in four out of five litters from dams inoculated 
      around day 85 of gestation and in two out of three litters from dams inoculated 
      on day 72 of gestation. In the third group, inoculated around day 45 of 
      gestation, transplacental infection could not be demonstrated. Thirty-two (56%) 
      piglets from dams inoculated on day 85 of gestation and 14 (33%) piglets from 
      dams inoculated on day 72 of gestation, were transplacentally infected. Sixteen 
      (28%) and six (14%) piglets, respectively, in these groups became infected in the 
      perinatal period. Thirty-two (56%) piglets from dams inoculated on day 85 of 
      gestation were stillborn or died within a 6-8 weeks observation period, 29 being 
      stillborn or dying within the first two weeks of observation. Thirteen (30%) 
      piglets from dams inoculated on day 72 of gestation died within the two weeks 
      observation period. The duration of the viraemic phase varied considerably, from 
      one day to four weeks, for both dams and their offspring. Most frequently, PRRSV 
      was isolated from lung and/or tonsil tissues from dead and euthanized piglets 
      younger than 14 days of age. Histopathological investigations of piglets 
      typically revealed focal nonsuppurative inflammatory conditions, especially in 
      the lung and heart. In conclusion, the present results support the hypothesis, 
      that PRRSV infection of dams late in pregnancy has the greatest likelihood of 
      transplacental infection of fetuses.
FAU - Kranker, S
AU  - Kranker S
AD  - Danish Veterinary Institute for Virus Research, Kalvehave, Denmark.
FAU - Nielsen, J
AU  - Nielsen J
FAU - Bille-Hansen, V
AU  - Bille-Hansen V
FAU - Botner, A
AU  - Botner A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Brain/pathology/virology
MH  - Denmark
MH  - Female
MH  - Fetal Death/pathology/veterinary
MH  - Housing, Animal
MH  - Infectious Disease Transmission, Vertical/prevention & control/*veterinary
MH  - Necrosis
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology/pathology/transmission
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/isolation & 
      purification
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/*veterinary/virology
MH  - Swine
MH  - *Viral Vaccines
EDAT- 1998/07/01 00:00
MHDA- 1998/07/01 00:01
CRDT- 1998/07/01 00:00
PHST- 1998/07/01 00:00 [pubmed]
PHST- 1998/07/01 00:01 [medline]
PHST- 1998/07/01 00:00 [entrez]
AID - S0378-1135(98)00176-X [pii]
AID - 10.1016/s0378-1135(98)00176-x [doi]
PST - ppublish
SO  - Vet Microbiol. 1998 Mar 15;61(1-2):21-31. doi: 10.1016/s0378-1135(98)00176-x.