PMID- 9647834
OWN - NLM
STAT- MEDLINE
DCOM- 19980811
LR  - 20200724
IS  - 0099-2240 (Print)
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Linking)
VI  - 64
IP  - 7
DP  - 1998 Jul
TI  - Seasonal and spatial variability of bacterial and archaeal assemblages in the 
      coastal waters near Anvers Island, Antarctica.
PG  - 2585-95
AB  - A previous report of high levels of members of the domain Archaeal in Antarctic 
      coastal waters prompted us to investigate the ecology of Antarctic planktonic 
      prokaryotes. rRNA hybridization techniques and denaturing gradient gel 
      electrophoresis (DGGE) analysis of the bacterial V3 region were used to study 
      variation in Antarctic picoplankton assemblages. In Anvers Island nearshore 
      waters during late winter to early spring, the amounts of archaeal rRNA ranged 
      from 17.1 to 3.6% of the total picoplankton rRNA in 1996 and from 16.0 to 1.0% of 
      the total rRNA in 1995. Offshore in the Palmer Basin, the levels of archaeal rRNA 
      throughout the water column were higher (average, 24% of the total rRNA) during 
      the same period in 1996. The archaeal rRNA levels in nearshore waters followed a 
      highly seasonal pattern and markedly decreased during the austral summer at two 
      stations. There was a significant negative correlation between archaeal rRNA 
      levels and phytoplankton levels (as inferred from chlorophyll a concentrations) 
      in nearshore surface waters during the early spring of 1995 and during an 8-month 
      period in 1996 and 1997. In situ hybridization experiments revealed that 5 to 14% 
      of DAPI (4',6-diamidino-2-phenylindole)-stained cells were archaeal, 
      corresponding to 0.9 x 10(4) to 2.7 x 10(4) archaeal cells per ml, in late winter 
      1996 samples. Analysis of bacterial ribosomal DNA fragments by DGGE revealed that 
      the assemblage composition may reflect changes in water column stability, depth, 
      or season. The data indicate that changes in Antarctic seasons are accompanied by 
      significant shifts in the species composition of bacterioplankton assemblages and 
      by large decrease in the relative proportion of archaeal rRNA in the nearshore 
      water column.
FAU - Murray, A E
AU  - Murray AE
AD  - Marine Science Institute, University of California, Santa Barbara 93106, USA.
FAU - Preston, C M
AU  - Preston CM
FAU - Massana, R
AU  - Massana R
FAU - Taylor, L T
AU  - Taylor LT
FAU - Blakis, A
AU  - Blakis A
FAU - Wu, K
AU  - Wu K
FAU - DeLong, E F
AU  - DeLong EF
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
RN  - 0 (RNA, Archaeal)
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Ribosomal)
SB  - IM
MH  - Antarctic Regions
MH  - Archaea/*isolation & purification/metabolism
MH  - Bacteria/*isolation & purification/metabolism
MH  - In Situ Hybridization
MH  - Marine Biology
MH  - Nucleic Acid Hybridization
MH  - RNA, Archaeal/*analysis/metabolism
MH  - RNA, Bacterial/*analysis/metabolism
MH  - RNA, Ribosomal/*metabolism
MH  - *Seasons
MH  - Water Microbiology
PMC - PMC106430
EDAT- 1998/07/02 00:00
MHDA- 1998/07/02 00:01
PMCR- 1998/07/01
CRDT- 1998/07/02 00:00
PHST- 1998/07/02 00:00 [pubmed]
PHST- 1998/07/02 00:01 [medline]
PHST- 1998/07/02 00:00 [entrez]
PHST- 1998/07/01 00:00 [pmc-release]
AID - 1669 [pii]
AID - 10.1128/AEM.64.7.2585-2595.1998 [doi]
PST - ppublish
SO  - Appl Environ Microbiol. 1998 Jul;64(7):2585-95. doi: 
      10.1128/AEM.64.7.2585-2595.1998.