PMID- 9648560
OWN - NLM
STAT- MEDLINE
DCOM- 19980716
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 104
IP  - 1
DP  - 1998 Jul 1
TI  - The ABL/BCR fusion gene on chromosome 9 in Ph-negative chronic myelogenous 
      leukemia: a case for vigilance in fluorescence in situ hybridization 
      interpretation.
PG  - 57-60
AB  - We report cytogenetic, fluorescence in situ hybridization (FISH), and molecular 
      analysis in a case of Ph-negative chronic myelogenous leukemia patient with 
      ABL/BCR fusion gene on chromosome 9 and a disparate FISH signal pattern using two 
      commercially available bcr/abl probes (Vysis, Inc. and Oncor, Inc.). Cytogenetic 
      analysis revealed a 46,XX normal female karyotype. FISH studies using Vysis LSI 
      bcr/abl probe in interphase cells demonstrated a BCR/ABL fusion pattern, similar 
      to that of m-BCR/ABL fusion found in acute lymphoblastic leukemia. However, 
      examination of metaphases revealed the ABL/BCR fusion signal on one of the 
      chromosomes 9, an ABL signal on the other chromosome 9, and two BCR signals of 
      different sizes on each of the chromosomes 22. Subsequently, a FISH study with 
      the Oncor major (M)-bcr/abl translocation probe confirmed the ABL/BCR fusion 
      signal on chromosome 9 in addition to an ABL signal and a BCR signal located on 
      chromosomes 9 and 22, respectively. Molecular studies (RT-PCR) revealed a 
      rearrangement of the M-BCR region and expression of a chimeric bcr/abl mRNA of 
      b3a2 configuration. This case suggests that it is imperative to have a full 
      understanding of both the capabilities and the limitations of bcr/abl 
      translocation probes and that FISH interphase signals should be confirmed on 
      metaphase spreads for accurate diagnosis.
FAU - Hsu, W T
AU  - Hsu WT
AD  - Department of Pediatrics, Rush-Presbyterian-St. Luke's Medical Center, Rush 
      Medical College, Chicago, IL 60612, USA.
FAU - Preisler, H
AU  - Preisler H
FAU - Szego, K
AU  - Szego K
FAU - Sprudzs, R
AU  - Sprudzs R
FAU - Gao, X Z
AU  - Gao XZ
LA  - eng
PT  - Case Reports
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Chromosomes, Human, Pair 9/*genetics
MH  - Diagnosis, Differential
MH  - Female
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/diagnosis/epidemiology/*genetics
MH  - Leukemia, Myeloid, Chronic, Atypical, BCR-ABL 
      Negative/diagnosis/epidemiology/*genetics
MH  - Middle Aged
MH  - Observer Variation
MH  - Translocation, Genetic
EDAT- 1998/07/02 00:00
MHDA- 1998/07/02 00:01
CRDT- 1998/07/02 00:00
PHST- 1998/07/02 00:00 [pubmed]
PHST- 1998/07/02 00:01 [medline]
PHST- 1998/07/02 00:00 [entrez]
AID - S0165460897004305 [pii]
AID - 10.1016/s0165-4608(97)00430-5 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1998 Jul 1;104(1):57-60. doi: 
      10.1016/s0165-4608(97)00430-5.