PMID- 9649132
OWN - NLM
STAT- MEDLINE
DCOM- 19980713
LR  - 20190515
IS  - 0007-0920 (Print)
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Linking)
VI  - 77
IP  - 12
DP  - 1998 Jun
TI  - Is chromosome 9 loss a marker of disease recurrence in transitional cell 
      carcinoma of the urinary bladder?
PG  - 2193-8
AB  - Investigation of transitional cell carcinoma of the urinary bladder (TCC) 
      patients classified by recurrence and/or progression has demonstrated that loss 
      of chromosome 9, as detected by FISH analysis of the pericentromeric classical 
      satellite marker at 9q12, occurs early. A total of 105 TCCs from 53 patients were 
      analysed in situ by two independent observers for loss of chromosome 9 using 
      quantitative fluorescence in situ hybridization (FISH). All 53 primary tumours 
      were evaluated for chromosomes 9, 7 and 17. Normal ranges for chromosomal copy 
      number were defined for normal skin epidermis and bladder epithelium. Values for 
      chromosome 9 copy number outwith the range 1.51-2.10 (mean +/- 3 x s.d. of normal 
      values) were significantly abnormal. Twenty-five TCCs were detected with 
      consistent monosomic scores. Of 89 TCCs, in which multiple tumour areas were 
      analysed, 85 tumours (96%) demonstrated the same chromosome 9 copy number in all 
      areas (2-6) analysed; only three tumours demonstrated heterogeneity for this 
      locus. A total of 36% (12 out of 33) of patients with subsequent disease 
      recurrence demonstrated loss of chromosome 9 in their primary and all subsequent 
      TCCs analysed. Only a single patient (n = 20) with non-recurrent TCC showed loss 
      of chromosome 9 (P = 0.0085). Of 53 primary tumours, eight showed significant 
      elevation of chromosome 17. Of these patients, six demonstrated elevation in 
      chromosome 7 copy number. No abnormalities were observed in non-recurrent 
      patients. This study describes rapid quantitation of chromosomal copy number by 
      FISH using a pericentromeric probe for chromosome 9 in TCC of the urinary 
      bladder. Routinely fixed and processed material was evaluated without 
      disaggregation. Strict quality control of FISH demonstrated that this technique 
      was reproducible in a clinical environment and could be used to detect genetic 
      changes relevant to patient outcome. It is proposed that loss of chromosome 9 
      from primary TCC of the urinary bladder identified patients at high risk of 
      recurrence and possible progression.
FAU - Bartlett, J M
AU  - Bartlett JM
AD  - Glasgow University Department of Surgery, Glasgow Royal Infirmary, UK.
FAU - Watters, A D
AU  - Watters AD
FAU - Ballantyne, S A
AU  - Ballantyne SA
FAU - Going, J J
AU  - Going JJ
FAU - Grigor, K M
AU  - Grigor KM
FAU - Cooke, T G
AU  - Cooke TG
LA  - eng
PT  - Journal Article
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Transitional Cell/*genetics/pathology
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 17
MH  - Chromosomes, Human, Pair 7
MH  - *Chromosomes, Human, Pair 9
MH  - Disease Progression
MH  - Female
MH  - Genetic Markers
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Recurrence, Local/*genetics/pathology
MH  - Urinary Bladder Neoplasms/*genetics/pathology
PMC - PMC2150395
EDAT- 1998/07/02 00:00
MHDA- 1998/07/02 00:01
CRDT- 1998/07/02 00:00
PHST- 1998/07/02 00:00 [pubmed]
PHST- 1998/07/02 00:01 [medline]
PHST- 1998/07/02 00:00 [entrez]
AID - 10.1038/bjc.1998.365 [doi]
PST - ppublish
SO  - Br J Cancer. 1998 Jun;77(12):2193-8. doi: 10.1038/bjc.1998.365.