PMID- 9651380
OWN - NLM
STAT- MEDLINE
DCOM- 19980806
LR  - 20210209
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 273
IP  - 28
DP  - 1998 Jul 10
TI  - Adrenomedullin gene expression is developmentally regulated and induced by 
      hypoxia in rat ventricular cardiac myocytes.
PG  - 17787-92
AB  - Adrenomedullin is a recently discovered hypotensive peptide that is expressed in 
      a variety of cell and tissue types. Using the technique of differential display, 
      the adrenomedullin gene was observed to be differentially expressed in developing 
      rat heart. Reverse transcription-polymerase chain reaction analysis revealed that 
      the level of adrenomedullin mRNA was significantly higher in adult ventricular 
      cardiac muscle as compared with embryonic day 17 ventricular cardiac muscle. 
      Adrenomedullin receptor mRNA was constitutively expressed throughout development 
      of the ventricular heart. Two potential hypoxia-inducible factor-1 (HIF-1) 
      consensus binding sites were identified in the mouse adrenomedullin promoter at 
      -1095 and -770 nucleotides from the transcription start site. Exposure of 
      cultured adult rat ventricular cardiac myocytes to hypoxia (1% O2) resulted in a 
      significant, time-dependent increase in adrenomedullin mRNA levels. Transfection 
      studies revealed that the 5'-flanking sequence of adrenomedullin was capable of 
      mediating a hypoxia-inducible increase in transcription. Mutation of the putative 
      HIF-1 consensus binding sites revealed that the major regulatory sequence that 
      mediates the hypoxia-inducible transcriptional response is located at -1095. 
      These data demonstrate that the adrenomedullin gene is developmentally regulated 
      in ventricular cardiomyocytes, that adrenomedullin transcription can be induced 
      by hypoxia, and that this response is primarily mediated by HIF-1 consensus sites 
      in the adrenomedullin promoter.
FAU - Cormier-Regard, S
AU  - Cormier-Regard S
AD  - Department of Biochemistry and Molecular Biology, Louisiana State University 
      Medical Center, New Orleans, Louisiana 70112, USA.
FAU - Nguyen, S V
AU  - Nguyen SV
FAU - Claycomb, W C
AU  - Claycomb WC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (DNA Primers)
RN  - 0 (Peptides)
RN  - 0 (RNA, Messenger)
RN  - 148498-78-6 (Adrenomedullin)
SB  - IM
MH  - Adrenomedullin
MH  - Animals
MH  - Base Sequence
MH  - Cell Hypoxia/*genetics
MH  - Cells, Cultured
MH  - DNA Primers
MH  - *Gene Expression Regulation, Developmental
MH  - Heart Ventricles/cytology/*metabolism
MH  - Mice
MH  - Mutagenesis, Site-Directed
MH  - Peptides/*genetics
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Transcription, Genetic
EDAT- 1998/07/04 00:00
MHDA- 1998/07/04 00:01
CRDT- 1998/07/04 00:00
PHST- 1998/07/04 00:00 [pubmed]
PHST- 1998/07/04 00:01 [medline]
PHST- 1998/07/04 00:00 [entrez]
AID - S0021-9258(18)80556-7 [pii]
AID - 10.1074/jbc.273.28.17787 [doi]
PST - ppublish
SO  - J Biol Chem. 1998 Jul 10;273(28):17787-92. doi: 10.1074/jbc.273.28.17787.