PMID- 9652788
OWN - NLM
STAT- MEDLINE
DCOM- 19980710
LR  - 20190720
IS  - 0304-3835 (Print)
IS  - 0304-3835 (Linking)
VI  - 128
IP  - 1
DP  - 1998 Jun 5
TI  - Caspase proteases mediate apoptosis induced by anticancer agent preactivated 
      MC540 in human tumor cell lines.
PG  - 11-22
AB  - The molecular events involved in tumor cell death induced by novel photoproducts 
      of merocyanine 540 (pMC540) are poorly understood. Using HL60 leukemia and M14 
      melanoma cell lines we investigated the role of the apoptotic pathway in 
      pMC540-mediated cell death. Tumor cells exposed to pMC540 showed cell size 
      shrinkage and an increase in the sub-diploid DNA content. A loss of membrane 
      phospholipid asymmetry associated with apoptosis was induced by pMC540 in both 
      tumor cell lines as evidenced by the externalization of phosphatidylserine. A 
      dose-dependent increase in caspase-3 protease activity suppressed by the 
      tetrapeptide inhibitor DEVD-CHO was observed in both cell lines. Western blot 
      analysis of poly (ADP-ribose) polymerase, a caspase substrate, showed the 
      classical cleavage pattern (116 to 89 kDa) associated with apoptosis in 
      pMC540-treated cell lysates. Furthermore, caspase inhibition blocked the 
      externalization of membrane PS, indicating that the loss of membrane phospholipid 
      asymmetry is a downstream event of caspase activation. These findings demonstrate 
      that tumor cell death induced by pMC540 is mediated by caspase proteases.
FAU - Pervaiz, S
AU  - Pervaiz S
AD  - Department of Physiology, Faculty of Medicine, National University of Singapore, 
      Singapore. shazib@pacific.net.sg
FAU - Hirpara, J L
AU  - Hirpara JL
FAU - Clement, M V
AU  - Clement MV
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Cancer Lett
JT  - Cancer letters
JID - 7600053
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Phosphatidylserines)
RN  - 0 (Pyrimidinones)
RN  - 58823-12-4 (merocyanine dye)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
SB  - IM
MH  - Apoptosis
MH  - Cell Size
MH  - Cysteine Endopeptidases/*pharmacology
MH  - DNA, Neoplasm/analysis
MH  - Enzyme Activation
MH  - Humans
MH  - Leukemia
MH  - Melanoma
MH  - Phosphatidylserines/metabolism
MH  - Pyrimidinones/*pharmacology
MH  - Tumor Cells, Cultured
EDAT- 1998/07/04 00:00
MHDA- 1998/07/04 00:01
CRDT- 1998/07/04 00:00
PHST- 1998/07/04 00:00 [pubmed]
PHST- 1998/07/04 00:01 [medline]
PHST- 1998/07/04 00:00 [entrez]
AID - S0304-3835(98)00021-4 [pii]
AID - 10.1016/s0304-3835(98)00021-4 [doi]
PST - ppublish
SO  - Cancer Lett. 1998 Jun 5;128(1):11-22. doi: 10.1016/s0304-3835(98)00021-4.