PMID- 9652809
OWN - NLM
STAT- MEDLINE
DCOM- 19980716
LR  - 20190909
IS  - 0955-3002 (Print)
IS  - 0955-3002 (Linking)
VI  - 73
IP  - 5
DP  - 1998 May
TI  - Estimate of true incomplete exchanges using fluorescence in situ hybridization 
      with telomere probes.
PG  - 521-7
AB  - PURPOSE: To study the frequency of true incomplete exchanges in radiation-induced 
      chromosome aberrations. MATERIALS AND METHODS: Human lymphocytes were exposed to 
      2 Gy and 5 Gy of gamma-rays. Chromosome aberrations were studied using the 
      fluorescence in situ hybridization (FISH) technique with whole 
      chromosome-specific probes, together with human telomere probes. Chromosomes 2 
      and 4 were chosen in the present study. RESULTS: The percentage of incomplete 
      exchanges was 27% when telomere signals were not considered. After excluding 
      false incomplete exchanges identified by the telomere signals, the percentage of 
      incomplete exchanges decreased to 11%. Since telomere signals appear on about 82% 
      of the telomeres, the percentage of true incomplete exchanges should be even 
      lower and was estimated to be 3%. This percentage was similar for chromosomes 2 
      and 4 and for doses of both 2 Gy and 5 Gy. CONCLUSIONS: The percentage of true 
      incomplete exchanges is significantly lower in gamma-irradiated human lymphocytes 
      than the frequencies reported in the literature.
FAU - Wu, H
AU  - Wu H
AD  - NASA Johnson Space Center, Radiation Biophysics Laboratory, and Kelsey-Seybol 
      Clinic, Houston, Texas 77058, USA.
FAU - George, K
AU  - George K
FAU - Yang, T C
AU  - Yang TC
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Int J Radiat Biol
JT  - International journal of radiation biology
JID - 8809243
RN  - 0 (DNA Probes)
SB  - IM
MH  - *Chromosome Aberrations
MH  - Chromosomes, Human/*radiation effects
MH  - DNA Probes
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/radiation effects/*ultrastructure
MH  - *Telomere
OTO - NASA
OT  - NASA Center JSC
OT  - NASA Discipline Radiation Health
FIR - Yang, T C
IR  - Yang TC
IRAD- JSC
EDAT- 1998/07/04 00:00
MHDA- 1998/07/04 00:01
CRDT- 1998/07/04 00:00
PHST- 1998/07/04 00:00 [pubmed]
PHST- 1998/07/04 00:01 [medline]
PHST- 1998/07/04 00:00 [entrez]
AID - 10.1080/095530098142068 [doi]
PST - ppublish
SO  - Int J Radiat Biol. 1998 May;73(5):521-7. doi: 10.1080/095530098142068.