PMID- 9652823
OWN - NLM
STAT- MEDLINE
DCOM- 19980730
LR  - 20190818
IS  - 0300-9475 (Print)
IS  - 0300-9475 (Linking)
VI  - 47
IP  - 6
DP  - 1998 Jun
TI  - Expression of interferon-gamma, interleukin-4 and interleukin-5 mRNA in the nasal 
      mucosal membrane of rats with allergic rhinitis.
PG  - 554-60
AB  - The production of immunoglobulin E (IgE) antibody is largely dependent on the 
      ratio between interleukin-4 (IL-4) (a T helper 2 (Th2)-type cytokine) and 
      interferon-gamma (IFN-gamma) (a T helper 1 (Th1)-type cytokine). Interleukin-5 
      (IL-5) (also a Th2-type cytokine) is an important eosinophil differentiation 
      factor and also co-stimulates B-cell growth and differentiation. The present 
      study was designed to evaluate and compare the expression of IFN-gamma, IL-4 and 
      IL-5 mRNA in the nasal mucosal membrane of sensitized Brown-Norway (BN) rats. 
      Fourteen BN rats were divided into two groups: non-sensitized (control) and 
      sensitized. The sensitized group was injected with ovalbumin (OA) 
      intraperitoneally on three consecutive days. Twenty-one days later, rats were 
      exposed to 1% OA aerosol. Twenty-four hours after exposure to aerosol, nasal 
      mucosa was extracted from both groups and reverse transcriptase-polymerase chain 
      reaction (RT-PCR) was performed. The densities of the bands of IL-4, IL-5 and 
      IFN-gamma mRNA were expressed as percentages against beta-actin mRNA. Our results 
      showed that the mean values for IL-4 and IL-5 mRNA were increased significantly 
      in sensitized rats compared with control rats. In contrast, the mean value for 
      IFN-gamma mRNA was significantly lower in sensitized rats compared with those of 
      the control group. Our data therefore suggest that sensitization of rat nasal 
      mucous membranes results in the predominant expression of Th2-type cytokines.
FAU - El-Naggar, M M
AU  - El-Naggar MM
AD  - Department of Otorhinolaryngology, Mie University, School of Medicine, Tsu, 
      Japan.
FAU - Ukai, K
AU  - Ukai K
FAU - Takeuchi, K
AU  - Takeuchi K
FAU - Sakakura, Y
AU  - Sakakura Y
LA  - eng
PT  - Journal Article
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Azo Compounds)
RN  - 0 (Coloring Agents)
RN  - 0 (Interleukin-5)
RN  - 0 (RNA, Messenger)
RN  - 207137-56-2 (Interleukin-4)
RN  - 2610-05-1 (pontamine sky blue)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 9006-59-1 (Ovalbumin)
RN  - I2ZWO3LS3M (Trypan Blue)
SB  - IM
MH  - Airway Resistance
MH  - Animals
MH  - Azo Compounds/metabolism
MH  - Blotting, Southern
MH  - Coloring Agents/metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Gene Expression
MH  - Histamine/metabolism
MH  - Immunoglobulin E/blood/immunology
MH  - Interferon-gamma/*biosynthesis/genetics
MH  - Interleukin-4/*biosynthesis/genetics
MH  - Interleukin-5/*biosynthesis/genetics
MH  - Male
MH  - Nasal Mucosa/*immunology
MH  - Nasal Obstruction
MH  - Ovalbumin/immunology
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger
MH  - Rats
MH  - Rats, Inbred BN
MH  - Rhinitis/*immunology
MH  - Trypan Blue
EDAT- 1998/07/04 00:00
MHDA- 1998/07/04 00:01
CRDT- 1998/07/04 00:00
PHST- 1998/07/04 00:00 [pubmed]
PHST- 1998/07/04 00:01 [medline]
PHST- 1998/07/04 00:00 [entrez]
AID - 10.1046/j.1365-3083.1998.00351.x [doi]
PST - ppublish
SO  - Scand J Immunol. 1998 Jun;47(6):554-60. doi: 10.1046/j.1365-3083.1998.00351.x.