PMID- 9652910
OWN - NLM
STAT- MEDLINE
DCOM- 19980901
LR  - 20151119
IS  - 1065-7541 (Print)
IS  - 1065-7541 (Linking)
VI  - 6
IP  - 3
DP  - 1998
TI  - Measurement of proliferation activities in human tumor models: a comparison of 
      flow cytometric methods.
PG  - 120-7
AB  - Proliferating cells in tumors may be of considerable relevance in cancer therapy. 
      Not only do such cells dictate the rate of tumor progression, but evidence exists 
      that they may also play an important role in the diagnosis and prognosis of tumor 
      regrowth. Consequently, the identification of this subset of cells in the overall 
      neoplastic cell population is of considerable importance. The aim of the present 
      investigations was to compare four flow cytometric methodologies commonly used to 
      study cell proliferation. These included nuclear antigen Ki67 detection, acridine 
      orange (AO) and bromodeoxyuridine (BrdUrd) staining, and percent S-phase 
      determinations. Three human tumor cell lines (HEp3, A549, H226) were examined in 
      various stages of growth. Further, a direct comparison was made of the 
      proliferation activities of HEp3 cells grown in culture or as xenografts in nude 
      mice. The results showed that of the techniques investigated, detection of the 
      nuclear antigen Ki67 may be most useful for marking proliferating tumor cells and 
      determining tumor growth fractions.
FAU - Keng, P C
AU  - Keng PC
AD  - Cancer Center, University of Rochester School of Medicine and Dentistry, New 
      York, USA.
FAU - Siemann, D W
AU  - Siemann DW
LA  - eng
GR  - CA11051/CA/NCI NIH HHS/United States
GR  - CA11198/CA/NCI NIH HHS/United States
GR  - CA36858/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Radiat Oncol Investig
JT  - Radiation oncology investigations
JID - 9437448
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Ki-67 Antigen)
SB  - IM
MH  - Animals
MH  - Biomarkers, Tumor/analysis
MH  - Cell Count
MH  - Cell Division
MH  - DNA Replication/genetics
MH  - DNA, Neoplasm/analysis
MH  - Female
MH  - Flow Cytometry/*methods
MH  - Humans
MH  - Ki-67 Antigen/analysis
MH  - Mice
MH  - Mice, Nude
MH  - Mitotic Index
MH  - Neoplasm Transplantation
MH  - Neoplasms, Experimental/chemistry/genetics/*pathology
MH  - S Phase/genetics
MH  - Transplantation, Heterologous
MH  - Tumor Cells, Cultured
EDAT- 1998/07/04 00:00
MHDA- 1998/07/04 00:01
CRDT- 1998/07/04 00:00
PHST- 1998/07/04 00:00 [pubmed]
PHST- 1998/07/04 00:01 [medline]
PHST- 1998/07/04 00:00 [entrez]
AID - 10.1002/(SICI)1520-6823(1998)6:3<120::AID-ROI2>3.0.CO;2-# [doi]
PST - ppublish
SO  - Radiat Oncol Investig. 1998;6(3):120-7. doi: 
      10.1002/(SICI)1520-6823(1998)6:3<120::AID-ROI2>3.0.CO;2-#.