PMID- 9653493
OWN - NLM
STAT- MEDLINE
DCOM- 19980915
LR  - 20200203
IS  - 0923-7534 (Print)
IS  - 0923-7534 (Linking)
VI  - 9
IP  - 5
DP  - 1998 May
TI  - Application of interphase cytogenetics for the detection of t(11;14)(q13;q32) in 
      mantle cell lymphomas.
PG  - 519-26
AB  - BACKGROUND: The chromosomal translocation t(11;14)(q13;q32) is the hallmark of 
      mantle cell lymphoma (MCL) in which it can be detected cytogenetically in about 
      75% of cases. The t(11;14) translocation juxtaposes the bcl-1 locus in chromosome 
      band 11q13 next to the IgH locus in chromosome band 14q32 and, thus, leads to 
      deregulation of the cell cycle regulatory protein cyclin D1, which is encoded by 
      the CCND1 gene localized at the telomeric border of the bcl-1-locus. MCL has the 
      worst prognosis of all low-grade non-Hodgkin's lymphomas (NHL). In some 
      instances, however, histopathologic differentiation between MCL and other 
      low-grade B-cell NHL is difficult. Therefore, detection of the t(11;14) 
      translocation is of essential diagnostic value for the risk-adjusted management 
      of patients with MCL. Unfortunately, chromosome analyses are frequently hampered 
      by the low yield and quality of tumor metaphases. As the 11q13 breakpoints are 
      scattered over a region of more than 120 kb the application of molecular genetic 
      techniques is also limited. PATIENTS AND METHODS: We established an interphase 
      fluorescence in situ hybridization (FISH) approach for the detection of the 
      t(11;14) translocation by use of a cosmid probe hybridizing to the IgH constant 
      region and a YAC spanning the bcl-1 region. Cells containing a t(11;14) 
      translocation show a colocalisation of the signals for IgH and bcl-1. Eight 
      control samples and 15 MCL specimens were investigated. RESULTS: According to our 
      control studies, samples containing more than 10% of cells with this signal 
      constellation can be diagnosed as carrying a clonal t(11;14) translocation. All 
      eleven MCL found to carry the t(11;14) translocation by chromosome analysis were 
      positive in our FISH assay. Additionally, two of four MCL lacking a clonal 
      t(11;14) translocation by chromosome analysis were shown to carry this aberration 
      in 14% and 37% of interphase nuclei. Southern blot data indicate that our FISH 
      assay reliably detects the t(11;14) translocation irrespective of the location of 
      the breakpoints within the bcl-1 region. CONCLUSIONS: The described interphase 
      FISH assay provides a reliable and routinely applicable tool for diagnosis of the 
      t(11;14) translocation.
FAU - Siebert, R
AU  - Siebert R
AD  - Department of Human Genetics, University of Kiel, Germany. 
      office@medgen.uni-kiel.de
FAU - Matthiesen, P
AU  - Matthiesen P
FAU - Harder, S
AU  - Harder S
FAU - Zhang, Y
AU  - Zhang Y
FAU - Borowski, A
AU  - Borowski A
FAU - Zuhlke-Jenisch, R
AU  - Zuhlke-Jenisch R
FAU - Plendl, H
AU  - Plendl H
FAU - Metzke, S
AU  - Metzke S
FAU - Joos, S
AU  - Joos S
FAU - Zucca, E
AU  - Zucca E
FAU - Weber-Matthiesen, K
AU  - Weber-Matthiesen K
FAU - Roggero, E
AU  - Roggero E
FAU - Grote, W
AU  - Grote W
FAU - Schlegelberger, B
AU  - Schlegelberger B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Ann Oncol
JT  - Annals of oncology : official journal of the European Society for Medical 
      Oncology
JID - 9007735
SB  - IM
MH  - Aged
MH  - *Chromosomes, Human, Pair 11
MH  - *Chromosomes, Human, Pair 14
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphoma, Non-Hodgkin/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Translocation, Genetic
EDAT- 1998/07/08 00:00
MHDA- 1998/07/08 00:01
CRDT- 1998/07/08 00:00
PHST- 1998/07/08 00:00 [pubmed]
PHST- 1998/07/08 00:01 [medline]
PHST- 1998/07/08 00:00 [entrez]
AID - S0923-7534(19)61009-4 [pii]
AID - 10.1023/a:1008242729509 [doi]
PST - ppublish
SO  - Ann Oncol. 1998 May;9(5):519-26. doi: 10.1023/a:1008242729509.