PMID- 9653956
OWN - NLM
STAT- MEDLINE
DCOM- 19980824
LR  - 20190831
IS  - 1387-2273 (Print)
IS  - 1387-2273 (Linking)
VI  - 708
IP  - 1-2
DP  - 1998 Apr 24
TI  - Liquid chromatographic separation of doxycycline and 4-epidoxycycline in a tissue 
      depletion study of doxycycline in turkeys.
PG  - 145-52
AB  - Liver and muscle tissue residues of doxycycline in turkeys were determined 
      following administration of 25 mg doxycycline x HCl/kg BW in the drinking water 
      under field conditions. Quantitation was performed using a validated HPLC method 
      with fluorescence detection. The method was able to separate doxycycline and its 
      4-epimer, 4-epidoxycycline. This epimer was found in both liver and muscle 
      tissue. The detection limits of the method were estimated at 1.2 ng/g and 1.0 
      ng/g of doxycycline in liver and muscle tissue, respectively, using a 
      signal-to-noise ratio of 3:1. The recovery of doxycycline was determined from 
      spiked tissues and was 63+/-3.8% and 66+/-3.1% for liver and muscle, respectively 
      (n = 6). Within-day and between-day imprecision, expressed as the R.S.D. was 
      below 7.4%. Linear calibration curves (r>0.997) were obtained in spiked liver 
      between 0 and 1500 ng/g and in spiked muscle between 0 and 500 ng/g. A good 
      stability of doxycycline was observed in liver samples after storage for 22 days 
      at -20 degrees C. The correlation between the residues in the liver and the 
      muscle was expressed as the correlation coefficient r and was 0.9884. The 
      depletion kinetics of doxycycline fitted a one-compartment model. The elimination 
      half-life (T1/2) of doxycycline was 77.7 h and 78.0 h in muscle and liver, 
      respectively. Furthermore, the residue depletion kinetics were used to establish 
      a withdrawal period in conformity with official guidelines. The withdrawal times 
      necessary to reach concentrations below maximum residue limits (MRLs), as imposed 
      by the EU, were 12 days and 17 days for liver and muscle tissue, respectively.
FAU - Croubels, S
AU  - Croubels S
AD  - Department of Pharmacology, Pharmacy and Toxicology, Faculty of Veterinary 
      Medicine, University of Ghent, Merelbeke, Belgium.
FAU - Vermeersch, H
AU  - Vermeersch H
FAU - De Backer, P
AU  - De Backer P
FAU - Santos, M D
AU  - Santos MD
FAU - Remon, J P
AU  - Remon JP
FAU - Van Peteghem, C
AU  - Van Peteghem C
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Chromatogr B Biomed Sci Appl
JT  - Journal of chromatography. B, Biomedical sciences and applications
JID - 9714109
RN  - 0 (Anti-Bacterial Agents)
RN  - N12000U13O (Doxycycline)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/administration & dosage/*isolation & 
      purification/pharmacokinetics
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Doxycycline/administration & dosage/*isolation & purification/pharmacokinetics
MH  - Drug Residues/isolation & purification
MH  - Liver/chemistry
MH  - Male
MH  - Muscle, Skeletal/chemistry
MH  - Reproducibility of Results
MH  - Stereoisomerism
MH  - *Turkeys
EDAT- 1998/07/08 00:00
MHDA- 1998/07/08 00:01
CRDT- 1998/07/08 00:00
PHST- 1998/07/08 00:00 [pubmed]
PHST- 1998/07/08 00:01 [medline]
PHST- 1998/07/08 00:00 [entrez]
AID - 10.1016/s0378-4347(97)00644-0 [doi]
PST - ppublish
SO  - J Chromatogr B Biomed Sci Appl. 1998 Apr 24;708(1-2):145-52. doi: 
      10.1016/s0378-4347(97)00644-0.