PMID- 9655178
OWN - NLM
STAT- MEDLINE
DCOM- 19990401
LR  - 20190818
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 183
IP  - 1-2
DP  - 1998 Jun
TI  - Association of myeloperoxidase with heparin: oxidative inactivation of proteins 
      on the surface of endothelial cells by the bound enzyme.
PG  - 55-61
AB  - Chromatography of human myeloperoxidase (MPO) on a heparin-agarose column 
      demonstrated a tight association of the protein with the resin. The 
      electrophoretic mobility of mixtures of MPO and heparin in polyacrylamide gels 
      under nondenaturing conditions was consistent with a strong interaction of the 
      cationic enzyme with the polyanionic polysaccharide. Purified MPO prebound to 
      bovine aorta endothelial cells (BAEC) and supplemented with hydrogen peroxide 
      dose- and time-dependently abrogated the interaction of coagulation factor IX 
      (FIX) with factor IX-binding protein (FIXBP) on the surface of BAEC reflecting 
      oxidative modification of the binding protein. This inactivation of FIXBP 
      required the presence of chloride implicating hypochlorite in the reaction. 
      Hypochlorite and activated neutrophils exerted a similar effect. The oxidative 
      modification of FIXBP was only partially dependent on the addition of hydrogen 
      peroxide and was abolished by exogenous heparin which displaced MPO from the cell 
      surface, emphasizing the functional differences between cell-bound and free 
      enzyme.
FAU - Daphna, E M
AU  - Daphna EM
AD  - Department of Biochemistry, The Faculty of Life Sciences, Tel Aviv University, 
      Israel.
FAU - Michaela, S
AU  - Michaela S
FAU - Eynat, P
AU  - Eynat P
FAU - Irit, A
AU  - Irit A
FAU - Rimon, S
AU  - Rimon S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Chlorides)
RN  - 0 (Membrane Proteins)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (factor IX-IXa receptor)
RN  - 0 (heparin-sepharose)
RN  - 712K4CDC10 (Hypochlorous Acid)
RN  - 9001-28-9 (Factor IX)
RN  - 9005-49-6 (Heparin)
RN  - 9012-36-6 (Sepharose)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.1.3.4 (Glucose Oxidase)
RN  - EC 1.11.1.7 (Peroxidase)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cells, Cultured
MH  - Chlorides/physiology
MH  - Endothelium, Vascular/cytology/*enzymology/*metabolism
MH  - Factor IX/drug effects/metabolism
MH  - Glucose Oxidase/metabolism
MH  - Heparin/*metabolism/pharmacology
MH  - Humans
MH  - Hydrogen Peroxide/pharmacology
MH  - Hypochlorous Acid/metabolism
MH  - Membrane Proteins/*metabolism
MH  - Oxidation-Reduction/drug effects
MH  - Peroxidase/*metabolism
MH  - Protein Binding
MH  - Receptors, Cell Surface/drug effects/metabolism
MH  - Sepharose/analogs & derivatives/metabolism
EDAT- 1998/07/09 00:00
MHDA- 1998/07/09 00:01
CRDT- 1998/07/09 00:00
PHST- 1998/07/09 00:00 [pubmed]
PHST- 1998/07/09 00:01 [medline]
PHST- 1998/07/09 00:00 [entrez]
AID - 10.1023/a:1006848730927 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 1998 Jun;183(1-2):55-61. doi: 10.1023/a:1006848730927.