PMID- 9655354
OWN - NLM
STAT- MEDLINE
DCOM- 19980929
LR  - 20190905
IS  - 0931-041X (Print)
IS  - 0931-041X (Linking)
VI  - 12
IP  - 4
DP  - 1998 May
TI  - Developmental changes in carbonic anhydrase II in the rat kidney.
PG  - 263-8
AB  - We examined the distribution and maturational changes of carbonic anhydrase I 
      (CAI) and carbonic anhydrase II (CAII) in microdissected nephron segments of 
      Sprague-Dawley rats. CAI and CAII proteins were measured by enzyme-linked 
      immunosorbent assay. CAI was not detected in any nephron segment in 7-week-old 
      rats. CAII was present in the collecting ducts, proximal tubules, and thick 
      ascending limbs of loop of Henle in 7-week-old rats. CAII contents were 
      significantly higher in the early proximal tubules (S1) than in second (S2) and 
      late (S3) portions of the proximal tubules, while the contents in S1 were less 
      than in cortical collecting ducts (CCD), outer stripe and inner stripes of the 
      outer medullary collecting ducts (OMCDo and OMCDi). CAII content in each of S1, 
      CCD, and OMCD of 1-week-old rats was only 14% or less of that of adults, but 
      increased steeply during the 2nd and 3rd weeks of life, reaching almost 40% at 3 
      weeks of age and 97% at 7 weeks. Our results indicate that CAII is present 
      throughout the entire nephron of the rat, and that CAII content in S1, CCD, and 
      OMCD increases exponentially during the first 7 weeks of life. Our data suggest 
      that the immature low levels of CAII may explain, at least in part, the limited 
      capacity of urinary acidification during neonatal life. Further studies are 
      necessary to establish the role of such changes in CAII content in acid-base 
      homeostasis during neonatal life.
FAU - Karashima, S
AU  - Karashima S
AD  - Department of Pediatrics, Kumamoto University School of Medicine, Kumamoto-city, 
      Japan.
FAU - Hattori, S
AU  - Hattori S
FAU - Ushijima, T
AU  - Ushijima T
FAU - Furuse, A
AU  - Furuse A
FAU - Nakazato, H
AU  - Nakazato H
FAU - Matsuda, I
AU  - Matsuda I
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Pediatr Nephrol
JT  - Pediatric nephrology (Berlin, Germany)
JID - 8708728
RN  - 0 (Isoenzymes)
RN  - 0 (Proteins)
RN  - EC 4.2.1.1 (Carbonic Anhydrases)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Carbonic Anhydrases/*metabolism
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Isoenzymes/*metabolism
MH  - Kidney/*enzymology/*growth & development
MH  - Kidney Tubules, Collecting/enzymology/growth & development
MH  - Kidney Tubules, Proximal/enzymology/growth & development
MH  - Male
MH  - Proteins/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 1998/07/09 00:00
MHDA- 1998/07/09 00:01
CRDT- 1998/07/09 00:00
PHST- 1998/07/09 00:00 [pubmed]
PHST- 1998/07/09 00:01 [medline]
PHST- 1998/07/09 00:00 [entrez]
AID - 10.1007/s004670050450 [doi]
PST - ppublish
SO  - Pediatr Nephrol. 1998 May;12(4):263-8. doi: 10.1007/s004670050450.