PMID- 9658724
OWN - NLM
STAT- MEDLINE
DCOM- 19980807
LR  - 20061115
IS  - 0390-6078 (Print)
IS  - 0390-6078 (Linking)
VI  - 83
IP  - 5
DP  - 1998 May
TI  - Conventional cytogenetics and FISH evaluation of chimerism after sex-mismatched 
      bone marrow transplantation (BMT) and donor leukocyte infusion (DLI).
PG  - 408-15
AB  - BACKGROUND AND OBJECTIVES: Sensitive and quantitative cytogenetic methods to 
      better assess the biological significance of post-BMT chimerism have been 
      recently developed. In this study, we compared the results of chimerism analysis 
      and evolution employing conventional cytogenetics and fluorescence in situ 
      hybridization (FISH) in 16 patients after sex-mismatched BMT, and in 5 patients 
      after donor lymphocyte infusion (DLI) to treat post-BMT relapse. DESIGN AND 
      METHODS: FISH studies were performed using separate digoxigenin labeled 
      centromeric DNA probes for the X (pDMX1) and Y (DYZ1/DYZ3) chromosomes. To this 
      purpose, different types of samples were used: bone marrow (BM) and peripheral 
      blood (PB) slides processed for conventional cytogenetics, and routine BM and PB 
      smears. RESULTS: Results of chimerism studies performed on different types of 
      samples showed no significant differences. No significant differences in the 
      ability to identify the sex of each cell with both pDMX1 and DYZ1/DYZ3 probes 
      were found and the results obtained from independent experiments showed a high 
      linear correlation. Chimerism analysis by FISH showed initial mixed chimerism 
      after BMT in 10 patients. Seven of these patients were also studied by 
      conventional cytogenetics and 2 of these showed mixed chimerism. Seven of the 
      former 10 patients evolved to complete donor chimera. 6 patients showed 
      cytogenetic or hematologic bone marrow relapse, 3 of which were preceded by mixed 
      chimaerism as revealed by FISH studies. FISH studies permitted an easy and 
      accurate monitorization of the response to DLI in 5 relapsed patients, showing an 
      increase in the proportion of donor cells in 4 patients as they reached a new 
      complete remission. INTERPRETATION AND CONCLUSIONS: Both FISH and conventional 
      cytogenetics are quantitative methods to assess chimerism. However, FISH is more 
      sensitive, accurate and can even be applied on routine BM and PB smears. 
      Furthermore, its combination with immunophenotyping approaches to quantify 
      chimerism on cell subpopulations, will help to clarify post-BMT chimerism 
      significance.
FAU - Diez-Martin, J L
AU  - Diez-Martin JL
AD  - Department of Hematology, Clinica Puerta de Hierro, Madrid, Spain. 
      jldiez@radonc.hggm.es
FAU - Llamas, P
AU  - Llamas P
FAU - Gosalvez, J
AU  - Gosalvez J
FAU - Lopez-Fernandez, C
AU  - Lopez-Fernandez C
FAU - Polo, N
AU  - Polo N
FAU - de la Fuente, M S
AU  - de la Fuente MS
FAU - Buno, I
AU  - Buno I
LA  - eng
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Haematologica
JT  - Haematologica
JID - 0417435
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Blood Donors
MH  - *Bone Marrow Transplantation
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - *Leukocyte Transfusion
MH  - Male
MH  - *Transplantation Chimera
EDAT- 1998/07/11 00:00
MHDA- 1998/07/11 00:01
CRDT- 1998/07/11 00:00
PHST- 1998/07/11 00:00 [pubmed]
PHST- 1998/07/11 00:01 [medline]
PHST- 1998/07/11 00:00 [entrez]
PST - ppublish
SO  - Haematologica. 1998 May;83(5):408-15.