PMID- 9659233
OWN - NLM
STAT- MEDLINE
DCOM- 19980727
LR  - 20190512
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 93
IP  - 4
DP  - 1998 Apr
TI  - Specific inhibition of immunoglobulin E-mediated allergic reaction using 
      antisense Fc epsilon RI alpha oligodeoxynucleotides.
PG  - 589-94
AB  - We have investigated the ability of an antisense immunoglobulin E (IgE) receptor 
      alpha-subunit oligodeoxynucleotide (Fc epsilon RI alpha ODN) specifically to 
      inhibit IgE-mediated allergic reactions in the mouse. Synthetic antisense Fc 
      epsilon RI alpha ODN dose-dependently inhibited passive cutaneous anaphylaxis and 
      histamine release from the mouse peritoneal mast cells (MPMC) activated by 
      anti-dinitrophenyl (DNP) IgE. Northern blot analysis showed that the mast cells 
      treated with antisense Fc epsilon RI alpha ODN exhibited no detectable levels of 
      L-histidine decarboxylase mRNA after anti-DNP IgE stimulation, whereas the cells 
      treated with sense Fc epsilon RI alpha ODN possessed significant amounts of this 
      mRNA. Examination of the elevation of cAMP levels in MPMC following the 
      activation with anti-DNP IgE demonstrated a significant rise in activated cells, 
      but not in the antisense Fc epsilon RI alpha ODN-treated cells. Moreover, 
      antisense Fc epsilon RI alpha ODN had a significant inhibitory effect on anti-DNP 
      IgE-induced tumour necrosis factor-alpha production. Our results demonstrated 
      that antisense Fc epsilon RI alpha ODN inhibited the IgE-mediated allergic 
      reaction in vivo and in vitro.
FAU - Kim, H M
AU  - Kim HM
AD  - Department of Oriental Pharmacy, College of Pharmacy, Wonkwang University, Iksan, 
      Chonbuk, South Korea.
FAU - Kim, K S
AU  - Kim KS
FAU - Lee, E H
AU  - Lee EH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Oligonucleotides, Antisense)
RN  - 0 (Receptors, IgE)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Cyclic AMP/analysis
MH  - Dose-Response Relationship, Immunologic
MH  - Histamine Release/immunology
MH  - Hypersensitivity/*immunology
MH  - *Immune Tolerance
MH  - Immunoglobulin E/*immunology
MH  - Mast Cells/chemistry/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oligonucleotides, Antisense/*immunology
MH  - Passive Cutaneous Anaphylaxis/immunology
MH  - Polymerase Chain Reaction
MH  - Receptors, IgE/*immunology
MH  - Tumor Necrosis Factor-alpha/biosynthesis
PMC - PMC1364139
EDAT- 1998/07/11 00:00
MHDA- 1998/07/11 00:01
PMCR- 1999/04/01
CRDT- 1998/07/11 00:00
PHST- 1998/07/11 00:00 [pubmed]
PHST- 1998/07/11 00:01 [medline]
PHST- 1998/07/11 00:00 [entrez]
PHST- 1999/04/01 00:00 [pmc-release]
AID - 10.1046/j.1365-2567.1998.00453.x [doi]
PST - ppublish
SO  - Immunology. 1998 Apr;93(4):589-94. doi: 10.1046/j.1365-2567.1998.00453.x.