PMID- 9660067
OWN - NLM
STAT- MEDLINE
DCOM- 19980924
LR  - 20190909
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 54
IP  - 1
DP  - 1998 Mar
TI  - Molecular variation in the nucleoprotein gene (ORF7) of the porcine reproductive 
      and respiratory syndrome virus (PRRSV).
PG  - 9-21
AB  - The nucleoprotein gene (ORF7) of 15 European isolates of porcine reproductive and 
      respiratory syndrome virus (PRRSV) was sequenced and compared with corresponding 
      sequences of other PRRSV isolates (2 European and 13 American) and one isolate 
      each of other arteriviruses (the lactate dehydrogenase elevating virus (LDV), the 
      simian haemorrhagic fever virus (SHFV) and the equine arteritis virus (EAV)). 
      Their phylogenetic relationships were established using neighbour-joining and 
      parsimony methods. Four lineages (PRRSV, LDV, SHFV and EAV) were discriminated. 
      Two genotypes of PRRSV, European and American, could be further identified. The 
      European genotype of PRRSV was highly conserved. Analysis of the nucleotide and 
      amino acid substitutions in PRRSV ORF7 revealed four stable regions, probably 
      conserved because of their requirement for nucleocapsid function and/or 
      structure. No constant mutations accumulation in the ORF7 could be determined 
      precisely when either synonymous or non-synonymous mutations were studied. 
      Passage of the European PRRSV in vivo had little influence on the ORF7 sequence: 
      only a small number of synonymous substitutions in ORF7 was detectable, 
      confirming its low variability.
FAU - Le Gall, A
AU  - Le Gall A
AD  - Centre National d'Etudes Veterinaires et Alimentaires, Unite de Virologie et 
      Immunologie Porcines, Ploufragan, France.
FAU - Legeay, O
AU  - Legeay O
FAU - Bourhy, H
AU  - Bourhy H
FAU - Arnauld, C
AU  - Arnauld C
FAU - Albina, E
AU  - Albina E
FAU - Jestin, A
AU  - Jestin A
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (Nucleoproteins)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Evolution, Molecular
MH  - *Genetic Variation
MH  - Molecular Sequence Data
MH  - Nucleoproteins/*genetics
MH  - Open Reading Frames
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - Sequence Homology, Amino Acid
MH  - Swine
MH  - Viral Proteins/*genetics
EDAT- 1998/07/11 00:00
MHDA- 1998/07/11 00:01
CRDT- 1998/07/11 00:00
PHST- 1998/07/11 00:00 [pubmed]
PHST- 1998/07/11 00:01 [medline]
PHST- 1998/07/11 00:00 [entrez]
AID - S0168170297001469 [pii]
AID - 10.1016/s0168-1702(97)00146-9 [doi]
PST - ppublish
SO  - Virus Res. 1998 Mar;54(1):9-21. doi: 10.1016/s0168-1702(97)00146-9.