PMID- 9660068
OWN - NLM
STAT- MEDLINE
DCOM- 19980924
LR  - 20190909
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 54
IP  - 1
DP  - 1998 Mar
TI  - Synthetic peptides of human papillomavirus type 18 E6 harboring HLA-A2.1 motif 
      can induce peptide-specific cytotoxic T-cells from peripheral blood mononuclear 
      cells of healthy donors.
PG  - 23-9
AB  - To identify cytotoxic T-cell (CTL) epitopes against human papillomavirus type 18 
      (HPV 18) E6 protein that might be useful for developing peptide-based vaccine 
      against HPV 18 infection, 18 peptides which possibly contain CTL epitopes were 
      selected on the basis of previously described human leukocyte antigen 
      (HLA)-A2.1-binding motif and chemically synthesized. In the binding assay of the 
      synthetic peptides, 8 out of 18 synthetic peptides enhanced the expression of 
      HLA-A2.1 molecules on T2 cell surface, which implies that these peptides were 
      able to bind the HLA molecules. Those peptides having good binding affinity to 
      HLA-A2.1 were tested for their ability to activate CTLs which were isolated from 
      peripheral blood mononuclear cells (PBMCs) of healthy blood donors and to kill 
      the target T2 cells pulsed with the same peptide. Five out of eight tested 
      peptides activated CTLs and killed the target cells.
FAU - Yoon, H
AU  - Yoon H
AD  - Virus/Oncology Research Unit, Korea Research Institute of Bioscience and 
      Biotechnology, KIST, Yusong, Taejon, South Korea.
FAU - Chung, M K
AU  - Chung MK
FAU - Min, S S
AU  - Min SS
FAU - Lee, H G
AU  - Lee HG
FAU - Yoo, W D
AU  - Yoo WD
FAU - Chung, K T
AU  - Chung KT
FAU - Jung, N P
AU  - Jung NP
FAU - Park, S N
AU  - Park SN
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (E6 protein, Human papillomavirus type 18)
RN  - 0 (HLA-A2 Antigen)
RN  - 0 (Oncogene Proteins, Viral)
RN  - 0 (Peptides)
SB  - IM
MH  - Binding Sites
MH  - Cell Line
MH  - Cells, Cultured
MH  - *DNA-Binding Proteins
MH  - HLA-A2 Antigen/*immunology
MH  - Humans
MH  - Leukocytes, Mononuclear/immunology
MH  - Oncogene Proteins, Viral/chemical synthesis/*immunology
MH  - Papillomaviridae/*immunology
MH  - Peptides/chemical synthesis/immunology
MH  - T-Lymphocytes, Cytotoxic/*immunology
EDAT- 1998/07/11 00:00
MHDA- 1998/07/11 00:01
CRDT- 1998/07/11 00:00
PHST- 1998/07/11 00:00 [pubmed]
PHST- 1998/07/11 00:01 [medline]
PHST- 1998/07/11 00:00 [entrez]
AID - S0168170298000082 [pii]
AID - 10.1016/s0168-1702(98)00008-2 [doi]
PST - ppublish
SO  - Virus Res. 1998 Mar;54(1):23-9. doi: 10.1016/s0168-1702(98)00008-2.