PMID- 9660427
OWN - NLM
STAT- MEDLINE
DCOM- 19980716
LR  - 20190707
IS  - 0015-0282 (Print)
IS  - 0015-0282 (Linking)
VI  - 70
IP  - 1
DP  - 1998 Jul
TI  - 45,X/46,XX mosaicism in patients with idiopathic premature ovarian failure.
PG  - 89-93
AB  - OBJECTIVE: To determine whether low-level 45,X/46,XX mosaicism may be present in 
      some women with premature ovarian failure (POF). DESIGN: Slide preparations were 
      made from lymphocyte cultures stimulated with phytohemagglutinin, and 
      fluorescence in situ hybridization (FISH) was then performed using a 
      biotin-labeled X-chromosome probe. SETTING: Cytogenetics laboratory. PATIENT(S): 
      Fifteen women with idiopathic POF, 20 age-matched controls, and an additional 10 
      older control women with normal reproductive histories. INTERVENTION(S): Blood 
      samples were collected. MAIN OUTCOME MEASURE(S): The number of X chromosomes 
      present in each cell. RESULT(S): In patients with POF, the percentage of cells 
      with a single X chromosome (mean, 5.50) was significantly greater than in the 
      controls of similar age (mean, 2.42). For control subjects, there appeared to be 
      a linear correlation between the number of cells with a single X chromosome and 
      age. CONCLUSION(S): Some cases of POF may be attributable to low-level 45,X/46,XX 
      mosaicism, which can be detected using FISH. In the lymphocytes from normal 
      women, the rate of accumulation of cells with a single X chromosome was 
      approximately 700 per 10(6) cells per year.
FAU - Devi, A S
AU  - Devi AS
AD  - Department of Obstetrics and Gynecology, University of Connecticut Health Center, 
      Farmington 06030-6140, USA.
FAU - Metzger, D A
AU  - Metzger DA
FAU - Luciano, A A
AU  - Luciano AA
FAU - Benn, P A
AU  - Benn PA
LA  - eng
PT  - Clinical Trial
PT  - Journal Article
PL  - United States
TA  - Fertil Steril
JT  - Fertility and sterility
JID - 0372772
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aging/physiology
MH  - Aneuploidy
MH  - Female
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization
MH  - Karyotyping
MH  - Menarche/physiology
MH  - Middle Aged
MH  - Mitosis
MH  - Mosaicism/*genetics
MH  - Primary Ovarian Insufficiency/*genetics
MH  - X Chromosome/*genetics
EDAT- 1998/07/11 00:00
MHDA- 1998/07/11 00:01
CRDT- 1998/07/11 00:00
PHST- 1998/07/11 00:00 [pubmed]
PHST- 1998/07/11 00:01 [medline]
PHST- 1998/07/11 00:00 [entrez]
AID - S0015-0282(98)00122-8 [pii]
AID - 10.1016/s0015-0282(98)00122-8 [doi]
PST - ppublish
SO  - Fertil Steril. 1998 Jul;70(1):89-93. doi: 10.1016/s0015-0282(98)00122-8.