PMID- 9661750
OWN - NLM
STAT- MEDLINE
DCOM- 19981022
LR  - 20181201
IS  - 0192-9763 (Print)
IS  - 0192-9763 (Linking)
VI  - 19
IP  - 4
DP  - 1998 Jul
TI  - Growth factors in tympanic membrane perforations.
PG  - 428-34
AB  - OBJECTIVE: Little is known about the arrested healing of chronic central tympanic 
      membrane perforations and the mechanism involved in this process. Some authors 
      have traced the failure to a growth factor deficiency at the perforation margin. 
      In addition, recently, several growth factors have been tried out to improve 
      tympanic membrane (TM) closure in animals. The authors sought to determine the 
      expression of some well-known growth factor peptides in normal human TM and in 
      TMs with a chronic central perforation. MATERIALS AND METHODS: Total TM specimens 
      were obtained from patients with a normal TM (N = 10) soon after death and from 
      patients with a chronic perforation (N = 20) undergoing myringoplasty with use of 
      an allograft TM. Formaldehyde solution-fixed TMs were analyzed after 
      immunohistochemical staining using highly purified monoclonal antibodies to 
      determine whether epidermal growth factor receptor (EGF-r), transforming growth 
      factor-alpha (TGF-alpha), basic fibroblast growth factor (b-FGF), or transforming 
      growth factor-beta 1 (TGF-beta 1) was expressed in the TMs. RESULTS: The 
      distribution pattern for EGF-r, TGF-alpha, and b-FGF was similar in perforated 
      and nonperforated TMs. In contrast to this, TGF-beta 1 staining was markedly 
      different in perforated and nonperforated TMs. No or minimal TGF-beta 1 was 
      observed in normal TMs, whereas TGF-beta 1 staining was prominent in perforated 
      membranes, mostly at the perforation border. CONCLUSIONS: The authors 
      experimental findings imply that EGF-r, b-FGF, and TGF-alpha expression are not 
      significantly different in TMs with and without a central chronic perforation. 
      However, for TGF-beta 1, the authors found an increased staining pattern in 
      perforated TMs when compared with that of normal TMs, and staining at the 
      fibrotic and scarred perforation margin was pronounced. Based on these findings, 
      the authors speculate on the possible role of TGF-beta 1 in the development of 
      the fibrotic scar at the perforation margin explaining the deficient healing 
      pattern of tympanic membranes in chronic otitis media. Possible clinical 
      implications for the future, including growth factor therapy, are discussed.
FAU - Somers, T
AU  - Somers T
AD  - University Department of Otolaryngology, Sint-Augustinus Hospital, Antwerp, 
      Belgium.
FAU - Goovaerts, G
AU  - Goovaerts G
FAU - Schelfhout, L
AU  - Schelfhout L
FAU - Peeters, S
AU  - Peeters S
FAU - Govaerts, P J
AU  - Govaerts PJ
FAU - Offeciers, E
AU  - Offeciers E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Am J Otol
JT  - The American journal of otology
JID - 7909513
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Transforming Growth Factor alpha)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Antibodies, Monoclonal
MH  - Chronic Disease
MH  - ErbB Receptors/*metabolism
MH  - Female
MH  - Fibroblast Growth Factor 2/*metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Middle Aged
MH  - Otitis Media/complications
MH  - Transforming Growth Factor alpha/*metabolism
MH  - Tympanic Membrane Perforation/etiology/*metabolism/pathology
EDAT- 1998/07/14 00:00
MHDA- 1998/07/14 00:01
CRDT- 1998/07/14 00:00
PHST- 1998/07/14 00:00 [pubmed]
PHST- 1998/07/14 00:01 [medline]
PHST- 1998/07/14 00:00 [entrez]
PST - ppublish
SO  - Am J Otol. 1998 Jul;19(4):428-34.