PMID- 9664910
OWN - NLM
STAT- MEDLINE
DCOM- 19980721
LR  - 20181201
IS  - 0022-3417 (Print)
IS  - 0022-3417 (Linking)
VI  - 184
IP  - 4
DP  - 1998 Apr
TI  - EGF-r gene copy number changes in renal cell carcinoma detected by fluorescence 
      in situ hybridization.
PG  - 424-9
AB  - Expression of epidermal growth factor receptor (EGF-r) is a frequent event in 
      renal cell carcinoma (RCC). To investigate the role of EGF-r gene copy number 
      changes related to EGF-r overexpression, 50 RCC specimens were examined by 
      fluorescence in situ hybridization (FISH) and immunohistochemistry. 
      Dual-labelling FISH with a repetitive pericentromeric probe for chromosome 7 and 
      a probe for the EGF-r gene (at 7p13) was performed to analyse the EGF-r copy 
      number in relation to chromosome 7 copy number on a cell-by-cell basis. Polysomy 
      7 was frequent in all histological types of RCC. Chromosome 7 polysomy was found 
      in 26 of 35 clear cell (74 per cent), nine of nine papillary, and three of three 
      chromophobe RCCs. EGF-r gene copy number was closely associated with the 
      chromosome 7 copy number on a cell-by-cell basis. No EGF-r gene amplifications 
      were found. EGF-r positivity was found in 37 of 50 cases (74 per cent) by 
      immunohistochemistry. EGF-r positivity was more common in clear cell (81 per 
      cent) than in papillary tumours (40 per cent; P = 0.029). Neither chromosome 7 
      nor EGF-r gene copy number was associated with EGF-r expression, indicating that 
      an increased gene dosage is not a mechanism of EGF-r overexpression in RCC.
FAU - Moch, H
AU  - Moch H
AD  - Institute of Pathology, University of Basel, Switzerland.
FAU - Sauter, G
AU  - Sauter G
FAU - Gasser, T C
AU  - Gasser TC
FAU - Bubendorf, L
AU  - Bubendorf L
FAU - Richter, J
AU  - Richter J
FAU - Presti, J C Jr
AU  - Presti JC Jr
FAU - Waldman, F M
AU  - Waldman FM
FAU - Mihatsch, M J
AU  - Mihatsch MJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Pathol
JT  - The Journal of pathology
JID - 0204634
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Neoplasm Proteins)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Carcinoma, Renal Cell/*genetics/metabolism/pathology
MH  - Chromosomes, Human, Pair 7
MH  - ErbB Receptors/*genetics/metabolism
MH  - *Gene Dosage
MH  - Gene Expression
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Ki-67 Antigen/metabolism
MH  - Kidney Neoplasms/*genetics/metabolism/pathology
MH  - Neoplasm Proteins/genetics/metabolism
EDAT- 1998/07/17 03:04
MHDA- 2000/06/20 09:00
CRDT- 1998/07/17 03:04
PHST- 1998/07/17 03:04 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/07/17 03:04 [entrez]
AID - 10.1002/(SICI)1096-9896(199804)184:4<424::AID-PATH1223>3.0.CO;2-A [pii]
AID - 10.1002/(SICI)1096-9896(199804)184:4<424::AID-PATH1223>3.0.CO;2-A [doi]
PST - ppublish
SO  - J Pathol. 1998 Apr;184(4):424-9. doi: 
      10.1002/(SICI)1096-9896(199804)184:4<424::AID-PATH1223>3.0.CO;2-A.