PMID- 9664973
OWN - NLM
STAT- MEDLINE
DCOM- 19981130
LR  - 20190914
IS  - 0934-0874 (Print)
IS  - 0934-0874 (Linking)
VI  - 11 Suppl 1
DP  - 1998
TI  - Establishment of chimerism in donor liver with recipient-type bone marrow cells 
      prior to liver transplantation produces marked suppression of allograft rejection 
      in rats.
PG  - S174-8
AB  - In this study, we investigated whether establishment of chimerism in donor liver 
      with recipient-type bone marrow cells (BMCs) prior to liver transplantation could 
      prolong the liver allograft survival. Donor female ACI rats were inoculated with 
      recipient-type BMCs of male LEW rats via the portal vein, with or without 
      irradiation as cytoablation, followed by intramuscular administration of FK506 
      for 5 days. At 1-2 months later, livers were harvested and transplanted into 
      naive female LEW rats. No immunosuppressants were used. Chimerism in donor rats 
      was confirmed by primers specific for the sex determinant Y chromosome of rats. 
      With livers from rats pretreated with recipient-type BMCs, survival of liver 
      allografts was significantly extended, irrespective of irradiation. These results 
      showed that modification of the donor liver by intraportal injection of 
      recipient-type BMCs and concomitant administration of FK506 prior to liver 
      transplantation prolonged liver allograft survival in rats.
FAU - Sanada, O
AU  - Sanada O
AD  - Second Department of Surgery, School of Medicine, Hiroshima University, Japan.
FAU - Fukuda, Y
AU  - Fukuda Y
FAU - Sumimoto, R
AU  - Sumimoto R
FAU - Hoshino, S
AU  - Hoshino S
FAU - Nishihara, M
AU  - Nishihara M
FAU - Kaneda, K
AU  - Kaneda K
FAU - Asahara, T
AU  - Asahara T
FAU - Dohi, K
AU  - Dohi K
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Transpl Int
JT  - Transplant international : official journal of the European Society for Organ 
      Transplantation
JID - 8908516
SB  - IM
MH  - Animals
MH  - *Bone Marrow Transplantation
MH  - Dendritic Cells
MH  - Female
MH  - Graft Rejection/*prevention & control
MH  - Graft Survival
MH  - *Liver Transplantation
MH  - Male
MH  - Preoperative Care
MH  - Rats
MH  - Rats, Inbred ACI
MH  - Rats, Inbred Lew
MH  - Survival Rate
MH  - *Transplantation Chimera
EDAT- 1998/07/17 00:00
MHDA- 1998/07/17 00:01
CRDT- 1998/07/17 00:00
PHST- 1998/07/17 00:00 [pubmed]
PHST- 1998/07/17 00:01 [medline]
PHST- 1998/07/17 00:00 [entrez]
AID - 10.1007/s001470050455 [doi]
PST - ppublish
SO  - Transpl Int. 1998;11 Suppl 1:S174-8. doi: 10.1007/s001470050455.