PMID- 9665376
OWN - NLM
STAT- MEDLINE
DCOM- 19980723
LR  - 20191102
IS  - 0022-2143 (Print)
IS  - 0022-2143 (Linking)
VI  - 132
IP  - 1
DP  - 1998 Jul
TI  - Chinese herbs as modulators of human mesangial cell proliferation: preliminary 
      studies.
PG  - 76-85
AB  - In the hope of identifying agents of therapeutic value in immunoglobulin A 
      nephropathy (IgA-N), we tested crude methanol extracts of 15 Chinese herbs for 
      their effect on human mesangial cel proliferation in vitro. The results indicated 
      that 7 out of the 15 crude extracts inhibited human mesangial cell proliferation 
      activated by interleukin-1beta and interleukin-6. The extracts and their median 
      inhibitory concentrations were as follows (in microg/ml): Selaginella tamariscina 
      (MLS-032), 56.0 +/- 2.0; Ixeris chinensis (MLS-033), 62.7 +/- 1.7; Polygonum 
      hypoleucum Ohwi (MLS-034), 25.0 +/- 1.5; Scutellaris rivularis (MLS-036), 39.6 
      +/- 1.1; Condonacanthus paucifiorus (MLS-042),63.6 +/- 2.6; Xanthium strumarium 
      (MLS-043), 42.8 +/- 1.3; Daemonoropus margaritae (MLS-044), 56.1 +/- 1.9. These 
      findings indicate that human mesangial cells were most sensitive to MLS-034 
      treatment. These herbs also decreased interleukin-1beta and tumor necrosis 
      factor-alpha production. Moreover, TNF-alpha mRNA expression was inhibited by 
      MLS-034. It is unlikely that cytotoxicity was involved, because no cell deaths 
      were observable. We hypothesize that the inhibitory mechanisms of these Chinese 
      herbs may be related to the impairments of gene expression and production of 
      cytokines in human mesangial cells. Plans are underway for the isolation of pure 
      compounds from these Chinese herbs and the elucidation of their mechanisms of 
      action.
FAU - Kuo, Y C
AU  - Kuo YC
AD  - National Research Institute of Chinese Medicine, Department of Pediatrics, 
      Veterans' General Hospital, Taipei, Taiwan, Republic of China.
FAU - Sun, C M
AU  - Sun CM
FAU - Tsai, W J
AU  - Tsai WJ
FAU - Ou, J C
AU  - Ou JC
FAU - Chen, W P
AU  - Chen WP
FAU - Lin, C Y
AU  - Lin CY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Lab Clin Med
JT  - The Journal of laboratory and clinical medicine
JID - 0375375
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Child
MH  - Dose-Response Relationship, Drug
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Glomerular Mesangium/cytology/*drug effects
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Interleukin-6/pharmacology
MH  - RNA, Messenger/analysis
MH  - Tumor Necrosis Factor-alpha/biosynthesis/genetics
EDAT- 1998/07/17 00:00
MHDA- 1998/07/17 00:01
CRDT- 1998/07/17 00:00
PHST- 1998/07/17 00:00 [pubmed]
PHST- 1998/07/17 00:01 [medline]
PHST- 1998/07/17 00:00 [entrez]
AID - S0022-2143(98)90029-4 [pii]
AID - 10.1016/s0022-2143(98)90029-4 [doi]
PST - ppublish
SO  - J Lab Clin Med. 1998 Jul;132(1):76-85. doi: 10.1016/s0022-2143(98)90029-4.