PMID- 9665995
OWN - NLM
STAT- MEDLINE
DCOM- 19981013
LR  - 20220318
IS  - 0095-1137 (Print)
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Linking)
VI  - 36
IP  - 8
DP  - 1998 Aug
TI  - Detection and semiquantitative analysis of human herpesvirus 8 DNA in specimens 
      from patients with Kaposi's sarcoma.
PG  - 2220-2
AB  - Kaposi's sarcoma (KS) is the most common neoplasm in patients with AIDS. 
      Epidemiologic evidence and the recent identification of herpesvirus-like DNA 
      sequences in patients with KS have suggested a role for viral agents in the 
      etiopathogenesis of this disease. It is unclear if these sequences are present in 
      all types of KS and if the copy number of these sequences has a correlation with 
      disease severity (staging). In order to clarify these issues, we retrospectively 
      analyzed, by PCR and Southern blotting, formalin-fixed, paraffin-embedded biopsy 
      specimens from 12 patients previously diagnosed with KS by histopathologic 
      examination of these specimens between the years of 1977 and 1996. We also 
      analyzed tissue samples from these patients taken from dermal sites without KS 
      lesions and control tissues from healthy subjects. Of the 12 patients, 6 had 
      classic KS, 5 had AIDS-associated KS, and 1 had the endemic type of KS. We tested 
      the specimens for other herpesviruses, including cytomegalovirus, human 
      herpesvirus 6 (HHV-6), HHV-7, HHV-8, and Epstein-Barr virus. Of 20 biopsy 
      specimens from patients previously diagnosed with KS, 19 were positive for HHV-8 
      sequences (95%), while PCR for the DNAs of other herpesvirus agents was negative. 
      Uninvolved tissue from these patients and control tissue from healthy subjects 
      gave negative results for all viruses. Semiquantitative analysis of Southern 
      blots showed higher levels of HHV-8 DNA in those patients with multicentric and 
      visceral involvement than in those patients with localized involvement. In 
      addition, in patients with localized skin disease, the nodular stage had higher 
      levels of HHV-8 DNA than the patch or plaque stages. Our data confirmed that 
      HHV-8 is involved in the etiopathogenesis of all types of KS and that there is a 
      correlation between HHV-8 DNA load and the severity and staging of this disease.
FAU - Mendez, J C
AU  - Mendez JC
AD  - Division of Infectious Diseases, Mayo Clinic, Rochester, Minnesota 55905, USA.
FAU - Procop, G W
AU  - Procop GW
FAU - Espy, M J
AU  - Espy MJ
FAU - Paya, C V
AU  - Paya CV
FAU - Smith, T F
AU  - Smith TF
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
RN  - 0 (DNA, Viral)
SB  - IM
MH  - AIDS-Related Opportunistic Infections/*virology
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Blotting, Southern
MH  - DNA, Viral/*analysis
MH  - Female
MH  - Herpesvirus 8, Human/genetics/*isolation & purification
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction/methods
MH  - Retrospective Studies
MH  - Sarcoma, Kaposi/pathology/*virology
MH  - Skin Neoplasms/pathology/*virology
PMC - PMC105018
EDAT- 1998/07/17 00:00
MHDA- 1998/07/17 00:01
PMCR- 1998/08/01
CRDT- 1998/07/17 00:00
PHST- 1998/07/17 00:00 [pubmed]
PHST- 1998/07/17 00:01 [medline]
PHST- 1998/07/17 00:00 [entrez]
PHST- 1998/08/01 00:00 [pmc-release]
AID - 0094 [pii]
AID - 10.1128/JCM.36.8.2220-2222.1998 [doi]
PST - ppublish
SO  - J Clin Microbiol. 1998 Aug;36(8):2220-2. doi: 10.1128/JCM.36.8.2220-2222.1998.