PMID- 9666100
OWN - NLM
STAT- MEDLINE
DCOM- 19980904
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 215
IP  - 1
DP  - 1998 Jul 17
TI  - Cloning and chromosomal localisation of the murine epidermal-type fatty acid 
      binding protein gene (Fabpe).
PG  - 123-30
AB  - We succeeded in cloning the gene encoding the murine epidermal-type fatty acid 
      binding protein (E-FABP). To avoid the screening of pseudogenes, the presence of 
      which was shown by PCR, we designed an intron-specific probe and screened a 
      bacterial artificial chromosome library from mouse embryonic stem cells. One of 
      the clones obtained was analysed by restriction with various enzymes and an 11-kb 
      EcoRI fragment with the complete gene was subcloned. The gene revealed the 
      canonical exon/intron FABP structure consisting of four exons (112, 173, 102 and 
      544bp, respectively) and three introns (2217, 327 and 546bp, respectively). The 
      exon sequences were identical with the cDNA encoding mouse E-FABP (Krieg, P., 
      Feil, S., Furstenberger, G., Bowden, T.G., 1993. Tumor-specific overexpression of 
      a novel keratinocyte lipid-binding protein. Identification and characterisation 
      of a cloned sequence activated during multistage carcinogenesis in mouse skin. J. 
      Biol. Chem. 268, 17362-17369). Of the 5' region, 2470bp were sequenced and 
      searched for transcription factor binding sites. Putative responsive elements 
      within the promoter region were identified that may be responsible for the wide 
      expression observed for E-FABP in mouse tissues. The 11-kb EcoRI fragment was 
      used to localise Fabpe on chromosome 3 in the region 3A1-3 by fluorescence 
      in-situ hybridisation.
FAU - Bleck, B
AU  - Bleck B
AD  - Department of Biochemistry, University of Munster, Wilhelm-Klemm-Str. 2, D-48149, 
      Munster, Germany.
FAU - Hohoff, C
AU  - Hohoff C
FAU - Binas, B
AU  - Binas B
FAU - Rustow, B
AU  - Rustow B
FAU - Dixkens, C
AU  - Dixkens C
FAU - Hameister, H
AU  - Hameister H
FAU - Borchers, T
AU  - Borchers T
FAU - Spener, F
AU  - Spener F
LA  - eng
SI  - GENBANK/AJ223066
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Carrier Proteins)
RN  - 0 (Fabp5 protein, mouse)
RN  - 0 (Fabp7 protein, mouse)
RN  - 0 (Fatty Acid-Binding Protein 7)
RN  - 0 (Fatty Acid-Binding Proteins)
RN  - 0 (Myelin P2 Protein)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Western
MH  - Carrier Proteins/analysis/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes/genetics
MH  - Cloning, Molecular
MH  - DNA/chemistry/genetics
MH  - Epidermis/*chemistry
MH  - Fatty Acid-Binding Protein 7
MH  - Fatty Acid-Binding Proteins
MH  - Genes/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Myelin P2 Protein/analysis/*genetics
MH  - *Neoplasm Proteins
MH  - *Nerve Tissue Proteins
MH  - Restriction Mapping
MH  - Sequence Analysis, DNA
MH  - Sequence Homology, Amino Acid
MH  - Tissue Distribution
EDAT- 1998/07/17 00:00
MHDA- 1998/07/17 00:01
CRDT- 1998/07/17 00:00
PHST- 1998/07/17 00:00 [pubmed]
PHST- 1998/07/17 00:01 [medline]
PHST- 1998/07/17 00:00 [entrez]
AID - S0378-1119(98)00262-5 [pii]
AID - 10.1016/s0378-1119(98)00262-5 [doi]
PST - ppublish
SO  - Gene. 1998 Jul 17;215(1):123-30. doi: 10.1016/s0378-1119(98)00262-5.