PMID- 9667682
OWN - NLM
STAT- MEDLINE
DCOM- 19980730
LR  - 20190515
IS  - 0007-0920 (Print)
IS  - 1532-1827 (Electronic)
IS  - 0007-0920 (Linking)
VI  - 77
IP  - 11
DP  - 1998 Jun
TI  - Comparison of chromosomal aberrations detected by fluorescence in situ 
      hybridization with clinical parameters, DNA ploidy and Ki 67 expression in renal 
      cell carcinoma.
PG  - 2003-7
AB  - To evaluate the significance of chromosomal aberrations in renal cell carcinoma, 
      fluorescence in situ hybridization (FISH) was used to determine its prevalence 
      and correlation with clinical parameters of malignancy. In addition, correlation 
      of chromosomal aberration with Ki 67 expression was analysed. We performed FISH 
      with chromosome-specific DNA probes, and the signal number of pericentromeric 
      sequences on chromosomes 3, 7, 9 and 17 was detected within interphase nuclei in 
      touch preparations from tumour specimen. The incidence of loss of chromosome 3 
      was significantly higher than those of chromosomes 7, 9 and 17 (P < 0.001, P = 
      0.03 and P < 0.001 respectively). Hyperdiploid aberration of chromosomes 3 and 17 
      was significantly correlated with tumour stage (P = 0.03, P = 0.02 respectively), 
      whereas hyperdiploid aberration of chromosome 9 was associated with nuclear grade 
      (P = 0.04). Disomy of chromosome 7 was correlated with venous involvement (P = 
      0.04). Ki 67 expression was significantly associated with hyperdiploid aberration 
      of chromosome 17 (P = 0.01), but not with aberration of chromosome 3. There was a 
      significant relationship between hyperdiploid aberration of chromosome 7 and Ki 
      67 expression (P = 0.01). In conclusions, gain of chromosome 17 may reflect 
      tumour development, and aberration of chromosome 7 may affect metastatic 
      potential of malignancy, whereas loss of chromosome 3 may be associated with 
      early stage of tumour development in renal cell carcinoma.
FAU - Wada, Y
AU  - Wada Y
AD  - Department of Urology, Shimane Medical University, Izumo, Japan.
FAU - Igawa, M
AU  - Igawa M
FAU - Shiina, H
AU  - Shiina H
FAU - Shigeno, K
AU  - Shigeno K
FAU - Yokogi, H
AU  - Yokogi H
FAU - Urakami, S
AU  - Urakami S
FAU - Yoneda, T
AU  - Yoneda T
FAU - Maruyama, R
AU  - Maruyama R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - England
TA  - Br J Cancer
JT  - British journal of cancer
JID - 0370635
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Ki-67 Antigen)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Carcinoma, Renal Cell/*genetics
MH  - *Chromosome Aberrations
MH  - DNA, Neoplasm/*analysis
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Ki-67 Antigen/*analysis
MH  - Kidney Neoplasms/*genetics
MH  - Male
MH  - Middle Aged
MH  - *Ploidies
PMC - PMC2150360
EDAT- 1998/07/17 00:00
MHDA- 1998/07/17 00:01
CRDT- 1998/07/17 00:00
PHST- 1998/07/17 00:00 [pubmed]
PHST- 1998/07/17 00:01 [medline]
PHST- 1998/07/17 00:00 [entrez]
AID - 10.1038/bjc.1998.332 [doi]
PST - ppublish
SO  - Br J Cancer. 1998 Jun;77(11):2003-7. doi: 10.1038/bjc.1998.332.