PMID- 9667734
OWN - NLM
STAT- MEDLINE
DCOM- 19980730
LR  - 20190512
IS  - 0143-3334 (Print)
IS  - 0143-3334 (Linking)
VI  - 19
IP  - 6
DP  - 1998 Jun
TI  - Comprehensive mutational scanning of the p53 coding region by two-dimensional 
      gene scanning.
PG  - 979-84
AB  - A comprehensive mutational scanning test for the p53 coding region based on 
      multiplex PCR and two-dimensional DNA electrophoresis was designed and evaluated. 
      In a 2-step multiplex PCR, the p53 coding region (exons 2-11) was amplified as a 
      single 8646-bp fragment by long-distance PCR in step one. This fragment served as 
      a template for the subsequent co-amplification of the individual exons in two 
      multiplex groups in step two. The multiplex products were then separated, first 
      on the basis of size in non-denaturant polyacrylamide gels and then on the basis 
      of sequence by denaturing gradient gel electrophoresis (DGGE). Primers for 
      optimal PCR, melting behavior and 2-D gel distribution were designed using a 
      recently developed computer program. The resulting two-dimensional gene scanning 
      (TDGS) test was evaluated by screening, in a blinded fashion, 29 coded DNA 
      samples from Li-Fraumeni syndrome patients with previously identified germline 
      mutations. All mutations were correctly detected. This assay provides an 
      accurate, cost-effective and non-radioactive method for simultaneous mutational 
      scanning of all p53 coding exons.
FAU - Rines, R D
AU  - Rines RD
AD  - Beth Israel Deaconess Medical Center, Harvard Institute of Medicine, Boston, MA 
      02115, USA.
FAU - van Orsouw, N J
AU  - van Orsouw NJ
FAU - Sigalas, I
AU  - Sigalas I
FAU - Li, F P
AU  - Li FP
FAU - Eng, C
AU  - Eng C
FAU - Vijg, J
AU  - Vijg J
LA  - eng
GR  - 1P30AG13314-02/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Carcinogenesis
JT  - Carcinogenesis
JID - 8008055
RN  - 0 (DNA Primers)
SB  - IM
MH  - Base Sequence
MH  - DNA Primers
MH  - Electrophoresis, Gel, Two-Dimensional/*methods
MH  - *Genes, p53
MH  - Humans
MH  - Li-Fraumeni Syndrome/genetics
MH  - Polymerase Chain Reaction
EDAT- 1998/07/17 00:00
MHDA- 1998/07/17 00:01
CRDT- 1998/07/17 00:00
PHST- 1998/07/17 00:00 [pubmed]
PHST- 1998/07/17 00:01 [medline]
PHST- 1998/07/17 00:00 [entrez]
AID - 10.1093/carcin/19.6.979 [doi]
PST - ppublish
SO  - Carcinogenesis. 1998 Jun;19(6):979-84. doi: 10.1093/carcin/19.6.979.