PMID- 9669669
OWN - NLM
STAT- MEDLINE
DCOM- 19990304
LR  - 20061115
IS  - 1045-2257 (Print)
IS  - 1045-2257 (Linking)
VI  - 22
IP  - 4
DP  - 1998 Aug
TI  - FISH characterization of head and neck carcinomas reveals that amplification of 
      band 11q13 is associated with deletion of distal 11q.
PG  - 312-20
AB  - In order to characterize homogeneously staining regions (HSR) and other 11q13 
      rearrangements identified cytogenetically, we performed fluorescence in situ 
      hybridization (FISH) using a CCND1 cosmid and five YAC clones spanning 
      chromosomal bands 11q13-14 on metaphase cells from 14 primary and one metastatic 
      head and neck carcinomas. At the cytogenetic level, a total of 17 HSR were 
      detected in ten cases: five were in derivative chromosomes 11 in band 11q13, and 
      12 were located in other derivative chromosomes. Other forms of 11q13 
      rearrangements were observed in five cases, whereas two cases had normal 
      chromosomes 11. FISH analysis demonstrated that all HSR but two were derived from 
      the 11q13 band. The size of the amplicon varied from case to case, but the 
      amplification always included the region covered by YAC 55G7, which contains the 
      CCND1 locus. The amplification of CCND1 was confirmed by use of a CCND1 cosmid. 
      We also showed that most of the cases (9 of 11) with 11q13 amplification had lost 
      material from distal 11q. The breakpoints were mapped by FISH and were shown to 
      cluster to the region between YACs 55G7 and 749G2. We conclude that loss of 
      gene(s) in distal 11q may be as important as amplification of genes in 11q13 for 
      the biological aggressiveness of head and neck carcinomas.
FAU - Jin, Y
AU  - Jin Y
AD  - Department of Clinical Genetics, University Hospital Lund, Sweden. 
      yuesheng.jin@klingen.lu.se
FAU - Hoglund, M
AU  - Hoglund M
FAU - Jin, C
AU  - Jin C
FAU - Martins, C
AU  - Martins C
FAU - Wennerberg, J
AU  - Wennerberg J
FAU - Akervall, J
AU  - Akervall J
FAU - Mandahl, N
AU  - Mandahl N
FAU - Mitelman, F
AU  - Mitelman F
FAU - Mertens, F
AU  - Mertens F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - *Chromosome Deletion
MH  - Chromosome Mapping
MH  - Chromosomes, Artificial, Yeast/genetics
MH  - Chromosomes, Human, Pair 11/*genetics
MH  - Female
MH  - Gene Amplification/*genetics
MH  - Head and Neck Neoplasms/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Male
MH  - Middle Aged
MH  - Sequence Homology, Nucleic Acid
EDAT- 1998/07/21 02:16
MHDA- 2000/06/20 09:00
CRDT- 1998/07/21 02:16
PHST- 1998/07/21 02:16 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/07/21 02:16 [entrez]
AID - 10.1002/(SICI)1098-2264(199808)22:4<312::AID-GCC7>3.0.CO;2-Y [pii]
PST - ppublish
SO  - Genes Chromosomes Cancer. 1998 Aug;22(4):312-20.