PMID- 9671128
OWN - NLM
STAT- MEDLINE
DCOM- 19980805
LR  - 20190723
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 213
IP  - 1
DP  - 1998 Apr 1
TI  - Stability of immunoglobulin E (IgE) in stored obstetric sera.
PG  - 99-101
AB  - OBJECTIVE: To determine the stability of immunoglobulin E levels in obstetric 
      sera. METHODS: AlaSTAT(R) and AlaTOP(R) (Diagnostic Products) were used to assay 
      total and specific IgE levels in obstetric sera collected in Memphis, TN and 
      Portland, OR. The samples were collected from the Collaborative Perinatal Project 
      (CPP) between 1959 and 1965 and stored at -20 degrees C. The assay results were 
      compared with IgE levels found in sera collected at the same locations for the 
      Calcium for Pre-eclampsia Prevention Study (CPEP) and stored since 1992 at -70 
      degrees C. The samples were also assayed for cockroach (CR) and mouse urine 
      specific IgE using the AlaSTAT(R) assay (Diagnostic Products). RESULTS: Total IgE 
      and specific IgE to CR and mouse urine were detectable in older and recent 
      samples. The median total IgE for the recent and older Portland samples was 26 
      IU/ml and 65 IU/ml, respectively. The median total IgE was identical (40 IU/ml) 
      in the recent and older Memphis samples. CONCLUSION: Long-term storage does not 
      diminish the ability to measure serum IgE. Levels of IgE in sera stored 32-37 
      years were equal to or greater than levels in sera stored for 5 years. reserved.
FAU - Henderson, C E
AU  - Henderson CE
AD  - Division of Epidemiology, Statistics and Prevention Research, National Institute 
      of Child Health and Development, NIH, Bethesda, MD, USA.
FAU - Ownby, D
AU  - Ownby D
FAU - Klebanoff, M
AU  - Klebanoff M
FAU - Levine, R J
AU  - Levine RJ
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9NEZ333N27 (Sodium)
SB  - IM
MH  - Animals
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Mice
MH  - Sodium/blood
EDAT- 1998/07/22 00:00
MHDA- 1998/07/22 00:01
CRDT- 1998/07/22 00:00
PHST- 1998/07/22 00:00 [pubmed]
PHST- 1998/07/22 00:01 [medline]
PHST- 1998/07/22 00:00 [entrez]
AID - S0022-1759(98)00014-3 [pii]
AID - 10.1016/s0022-1759(98)00014-3 [doi]
PST - ppublish
SO  - J Immunol Methods. 1998 Apr 1;213(1):99-101. doi: 10.1016/s0022-1759(98)00014-3.