PMID- 9671447
OWN - NLM
STAT- MEDLINE
DCOM- 20000628
LR  - 20170214
IS  - 0022-1554 (Print)
IS  - 0022-1554 (Linking)
VI  - 46
IP  - 8
DP  - 1998 Aug
TI  - Optimized fixation and storage conditions for FISH analysis of single-cell 
      suspensions.
PG  - 971-4
AB  - In our protocol to isolate and identify fetal cells in maternal peripheral blood, 
      antibody (Ab)-stained cells are preserved with paraformaldehyde (PF) before batch 
      flow cytometric sorting. However, PF fixation compromises the quality of 
      subsequent interphase fluorescence in situ hybridization (FISH). We therefore 
      examined the effect of PF concentrations and storage time in phosphate-buffered 
      saline (PBS) on the quality of FISH signals. Cells were analyzed for changes in 
      light scatter, morphology, and accessibility of target cell DNA. Fixation in 3% 
      PF for 1 hr was ideal for both flow cytometry and subsequent FISH detection. 
      However, beyond 10 days of storage, FISH quality deteriorated. (J Histochem 
      Cytochem 46:971-973, 1998)
FAU - Murrell-Bussell, S
AU  - Murrell-Bussell S
AD  - Departments of Microbiology and Immunology, Baylor College of Medicine, Houston, 
      Texas 77030, USA.
FAU - Nguyen, D
AU  - Nguyen D
FAU - Schober, W D
AU  - Schober WD
FAU - Scott, J
AU  - Scott J
FAU - Simpson, J L
AU  - Simpson JL
FAU - Elias, S
AU  - Elias S
FAU - Bischoff, F Z
AU  - Bischoff FZ
FAU - Lewis, D E
AU  - Lewis DE
LA  - eng
GR  - HD43203/HD/NICHD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Histochem Cytochem
JT  - The journal of histochemistry and cytochemistry : official journal of the 
      Histochemistry Society
JID - 9815334
RN  - 0 (Buffers)
RN  - 0 (Fixatives)
RN  - 0 (Polymers)
RN  - 1HG84L3525 (Formaldehyde)
RN  - Y19UC83H8E (paraform)
SB  - IM
MH  - Buffers
MH  - Fetus/cytology
MH  - Fixatives
MH  - Flow Cytometry
MH  - Formaldehyde
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukocytes, Mononuclear/*cytology
MH  - Polymers
MH  - *Specimen Handling
MH  - Time Factors
MH  - Tissue Fixation
EDAT- 1998/07/22 02:13
MHDA- 2000/07/06 11:00
CRDT- 1998/07/22 02:13
PHST- 1998/07/22 02:13 [pubmed]
PHST- 2000/07/06 11:00 [medline]
PHST- 1998/07/22 02:13 [entrez]
AID - 10.1177/002215549804600811 [doi]
PST - ppublish
SO  - J Histochem Cytochem. 1998 Aug;46(8):971-4. doi: 10.1177/002215549804600811.