PMID- 9671983
OWN - NLM
STAT- MEDLINE
DCOM- 19980924
LR  - 20220309
IS  - 0360-4012 (Print)
IS  - 0360-4012 (Linking)
VI  - 53
IP  - 2
DP  - 1998 Jul 15
TI  - Monocyte chemoattractant protein 1 is responsible for macrophage recruitment 
      following injury to sciatic nerve.
PG  - 260-7
AB  - Following injury to the peripheral nervous system, circulating 
      monocytes/macrophages are recruited to the damaged tissue, where they play vital 
      roles during both nerve degeneration and subsequent regeneration. Monocyte 
      chemoattractant protein-1 (MCP-1), a member of the C-C or beta-chemokine family, 
      is a powerful leukocyte recruitment/activation factor that is relatively specific 
      for monocytes/macrophages. Because these are the predominant leukocyte type 
      recruited by injured nerve, we hypothesized that upregulation of MCP-1 expression 
      is involved in recruitment of these cells. Indeed, assay of steady-state levels 
      of MCP-1 mRNA in rat sciatic nerve during tellurium-induced primary demyelination 
      indicated up-regulation of this chemokine with a peak after 3 days of tellurium 
      exposure, preceding the peak of accumulation of phagocytic macrophages (assayed 
      as lysozyme mRNA levels) by 6 days. Increasing levels of MCP-1 mRNA expression, 
      induced by increasing levels of tellurium exposure, resulted in corresponding 
      increases in subsequent recruitment of macrophages. In situ hybridization 
      suggested that MCP-1 mRNA was localized in Schwann cells. No expression of MIP-2, 
      which is a C-X-C or alpha-chemokine that is specific for recruitment of 
      neutrophils, was detected, consistent with the lack of recruitment of significant 
      numbers of these cells. In addition, we also investigated the response seen 
      following nerve transection (axonal degeneration and secondary demyelination with 
      no subsequent regeneration) and nerve crush (degeneration followed by 
      regeneration). In these latter two nerve injury models, there was also a marked, 
      early up-regulation of MCP-1 mRNA, with a time course that is compatible with a 
      role for this chemokine in macrophage recruitment. We conclude that MCP-1 is 
      involved in recruiting monocytes/macrophages to injured peripheral nerve and that 
      the specificity of leukocyte types recruited results from specificity of 
      chemokine production.
FAU - Toews, A D
AU  - Toews AD
AD  - Department of Biochemistry and Biophysics, Neuroscience Center, University of 
      North Carolina, Chapel Hill 27599-7250, USA. arrel@css.unc.edu
FAU - Barrett, C
AU  - Barrett C
FAU - Morell, P
AU  - Morell P
LA  - eng
GR  - ES-01104/ES/NIEHS NIH HHS/United States
GR  - HD-03110/HD/NICHD NIH HHS/United States
GR  - NS-11615/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - NQA0O090ZJ (Tellurium)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Chemokine CCL2/biosynthesis/*physiology
MH  - Demyelinating Diseases/chemically induced/pathology
MH  - Immunohistochemistry
MH  - In Situ Hybridization
MH  - Macrophages/metabolism/pathology/*physiology
MH  - Male
MH  - Nerve Crush
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sciatic Nerve/*injuries
MH  - Tellurium/toxicity
EDAT- 1998/07/22 02:14
MHDA- 2000/06/20 09:00
CRDT- 1998/07/22 02:14
PHST- 1998/07/22 02:14 [pubmed]
PHST- 2000/06/20 09:00 [medline]
PHST- 1998/07/22 02:14 [entrez]
AID - 10.1002/(SICI)1097-4547(19980715)53:2<260::AID-JNR15>3.0.CO;2-A [pii]
AID - 10.1002/(SICI)1097-4547(19980715)53:2<260::AID-JNR15>3.0.CO;2-A [doi]
PST - ppublish
SO  - J Neurosci Res. 1998 Jul 15;53(2):260-7. doi: 
      10.1002/(SICI)1097-4547(19980715)53:2<260::AID-JNR15>3.0.CO;2-A.