PMID- 9672608
OWN - NLM
STAT- MEDLINE
DCOM- 19980827
LR  - 20200324
IS  - 0304-8608 (Print)
IS  - 1432-8798 (Electronic)
IS  - 0304-8608 (Linking)
VI  - 142
IP  - 12
DP  - 1997
TI  - Effects of origin and state of differentiation and activation of 
      monocytes/macrophages on their susceptibility to porcine reproductive and 
      respiratory syndrome virus (PRRSV).
PG  - 2483-97
AB  - In this study, the susceptibility of porcine peripheral blood monocytes (BMo), 
      peritoneal macrophages (PM phi) and alveolar macrophages (AM phi) to PRRSV was 
      examined. To test the effect of differentiation and activation on their 
      susceptibility, AM phi and BMo were aged, cultivated in either adhesion or 
      suspension and treated with bacterial lipopolysaccharide (LPS) and phorbol 
      myristate acetate (PMA). It was found that freshly isolated PM phi and BMo were 
      non-permissive to PRRSV. PM phi remained refractory but a few BMo became 
      susceptible after 1 day cultivation. AM phi were permissive with a significant 
      increase of their susceptibility after one day cultivation. In a binding assay, 
      it was demonstrated that the attachment of biotinylated PRRSV to AM phi is much 
      more efficient than to PM phi and BMo. Two monoclonal antibodies (Mabs) 41D3 and 
      41D5 which block PRRSV infection of AM phi and are directed against a candidate 
      receptor for PRRSV only reacted with the cell membrane of AM phi. PMA treatment 
      of AM phi blocked PRRSV replication in the cells in a dose-dependent manner. The 
      blocking effect of PMA decreased after 9 h continuous pre-treatment and 
      diminished after 24 h continuous pre-treatment. PMA treatment did not affect the 
      binding of PRRSV and MAb 41D3 and 41D5 to AM phi. Direct or indirect treatment of 
      AM phi and BMo with LPS or cultivation in suspension did not significantly affect 
      their susceptibility. These results provide clear evidence that PRRSV has a 
      strongly restricted tropism for only some sub-populations of porcine 
      monocytes/macrophages and that some specific states of differentiation and 
      activation of monocytes/macrophages considerably affect their susceptibility.
FAU - Duan, X
AU  - Duan X
AD  - Laboratory of Veterinary Virology, University of Gent, Merelbeke, Belgium.
FAU - Nauwynck, H J
AU  - Nauwynck HJ
FAU - Pensaert, M B
AU  - Pensaert MB
LA  - eng
PT  - Journal Article
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Mitogens)
RN  - 6SO6U10H04 (Biotin)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/immunology
MH  - Antibodies, Viral/immunology
MH  - Biotin
MH  - Cell Adhesion
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Lipopolysaccharides/pharmacology
MH  - Macrophages, Alveolar/drug effects/*virology
MH  - Macrophages, Peritoneal/drug effects/*virology
MH  - Mitogens/pharmacology
MH  - Monocytes/drug effects/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Swine
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Virus Replication
PMC - PMC7086874
EDAT- 1997/01/01 00:00
MHDA- 1998/07/22 00:01
PMCR- 2020/03/23
CRDT- 1997/01/01 00:00
PHST- 1997/01/01 00:00 [pubmed]
PHST- 1998/07/22 00:01 [medline]
PHST- 1997/01/01 00:00 [entrez]
PHST- 2020/03/23 00:00 [pmc-release]
AID - 71422483 [pii]
AID - 10.1007/s007050050256 [doi]
PST - ppublish
SO  - Arch Virol. 1997;142(12):2483-97. doi: 10.1007/s007050050256.