PMID- 9673339
OWN - NLM
STAT- MEDLINE
DCOM- 19980810
LR  - 20071114
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 18
IP  - 3A
DP  - 1998 May-Jun
TI  - Comparison between fluorescence in situ hybridization and classical cytogenetics 
      in human tumors.
PG  - 1351-6
AB  - Cytogenetic and fluorescence in situ hybridization (FISH) studies permit analyses 
      of structural and numerical chromosome abnormalities. We compared modal 
      chromosome count in cancer cells by FISH and classical cytogenetics (CC). Our 
      cytogentic studies for chromosomes 3 and 17 on 134 human solid tumor cultures  inverted question mark57 
      small cell lung cancer (SCLC) and 77 non small cell lung cancers (NSCLC) inverted question mark, 
      indicated a high incidence of numerical changes in these tumors. Using 
      centromeric probes for chromosomes 3 and 17 we compared 15 tumor and 3 
      lymphoblastoid cultures, both by FISH (> 100 interphase nuclei) and by CC using 
      trypsin-Giemsa G banding (> 20 metaphases). Although some intra-tumor 
      heterogeneity was seen by FISH, we observed a high degree of concordancy (78%) in 
      modal count by the two techniques.
FAU - Virmani, A K
AU  - Virmani AK
AD  - Hamon Center for Therapeutic Oncology Research, University of Texas Southwestern 
      Medical Center at Dallas, Texas 75235-8593, USA.
FAU - Tonk, V S
AU  - Tonk VS
FAU - Gazdar, A F
AU  - Gazdar AF
LA  - eng
GR  - 1-P50-CA70907-01/CA/NCI NIH HHS/United States
GR  - N01-CN-45580-01/CN/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
SB  - IM
MH  - Carcinoma, Non-Small-Cell Lung/*genetics/pathology
MH  - Carcinoma, Small Cell/*genetics/pathology
MH  - Cell Line
MH  - Centromere
MH  - *Chromosome Aberrations
MH  - Chromosome Banding/methods
MH  - *Chromosome Disorders
MH  - *Chromosomes, Human, Pair 17
MH  - *Chromosomes, Human, Pair 3
MH  - Cytogenetics/methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Lung Neoplasms/*genetics/*pathology
MH  - Tumor Cells, Cultured
EDAT- 1998/07/23 00:00
MHDA- 1998/07/23 00:01
CRDT- 1998/07/23 00:00
PHST- 1998/07/23 00:00 [pubmed]
PHST- 1998/07/23 00:01 [medline]
PHST- 1998/07/23 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1998 May-Jun;18(3A):1351-6.