PMID- 9678716
OWN - NLM
STAT- MEDLINE
DCOM- 19980813
LR  - 20190826
IS  - 0145-2126 (Print)
IS  - 0145-2126 (Linking)
VI  - 22
IP  - 6
DP  - 1998 Jun
TI  - The hairy cell leukemia cell line Eskol spontaneously synthesizes tumor necrosis 
      factor-alpha and nitric oxide.
PG  - 501-7
AB  - Tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) exert a wide array 
      of immunoregulatory, partly related effects. We examined the production of these 
      two mediators by the human hairy cell leukemia cell line Eskol. Combined cell 
      lysate and supernatant of Eskol cells (0.5 x 10(6) cells ml(-1)) incubated for 18 
      h, contained a mean of 1.5 ng ml(-1) TNF-alpha. This spontaneous TNF-alpha 
      synthesis was enhanced by phorbol ester (PMA) and phytohemagglutinin (PHA) and 
      decreased by dexamethasone. Nitrite, the stable product of NO, accumulated in the 
      supernatant of Eskol cells after prolonged incubation. Maximal nitrite 
      concentrations (range: 0.8-3.5 microM at 2 x 10(6) cells ml(-1)) were detected 
      after 7 days of incubation. NO production was augmented by PHA and reduced by 
      PMA. The inhibitors of NO synthase N(G)-monomethyl-L-arginine (L-NMMA) and 
      aminoguanidine decreased NO synthesis. Simultaneous activation with the 
      proinflammatory cytokines, interferon-gamma, interleukin-1beta and TNF-alpha, 
      increased NO synthesis. These results suggest that NO production in Eskol cells 
      results from inducible NO synthase activity. This is the first direct 
      demonstration of NO formation in human lymphoid cells. The cell line, Eskol, may 
      serve as a model to study regulation of TNF-alpha and NO synthesis in human 
      B-cell leukemia.
FAU - Eigler, A
AU  - Eigler A
AD  - Division of Clinical Pharmacology, Medizinische Klinik, Klinikum Innenstadt of 
      the Ludwig-Maximilians-University, Munich, Germany.
FAU - Waller-Fontaine, K
AU  - Waller-Fontaine K
FAU - Moeller, J
AU  - Moeller J
FAU - Hartmann, G
AU  - Hartmann G
FAU - Hacker, U T
AU  - Hacker UT
FAU - Endres, S
AU  - Endres S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Leuk Res
JT  - Leukemia research
JID - 7706787
RN  - 0 (Interleukin-1)
RN  - 0 (Nitrates)
RN  - 0 (Phytohemagglutinins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Dexamethasone/pharmacology
MH  - Humans
MH  - Interferon-gamma/pharmacology
MH  - Interleukin-1/pharmacology
MH  - Leukemia, Hairy Cell/*metabolism
MH  - Nitrates/analysis
MH  - Nitric Oxide/*biosynthesis
MH  - Nitric Oxide Synthase/antagonists & inhibitors/pharmacology
MH  - Phytohemagglutinins/pharmacology
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Tumor Cells, Cultured/*metabolism
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/drug effects/pharmacology
EDAT- 1998/07/25 00:00
MHDA- 1998/07/25 00:01
CRDT- 1998/07/25 00:00
PHST- 1998/07/25 00:00 [pubmed]
PHST- 1998/07/25 00:01 [medline]
PHST- 1998/07/25 00:00 [entrez]
AID - S0145212698000149 [pii]
AID - 10.1016/s0145-2126(98)00014-9 [doi]
PST - ppublish
SO  - Leuk Res. 1998 Jun;22(6):501-7. doi: 10.1016/s0145-2126(98)00014-9.