PMID- 9679735
OWN - NLM
STAT- MEDLINE
DCOM- 19980804
LR  - 20220321
IS  - 1010-4283 (Print)
IS  - 1010-4283 (Linking)
VI  - 19
IP  - 4
DP  - 1998
TI  - Expression of two type II-like tumor hexokinase RNA transcripts in cancer cell 
      lines.
PG  - 253-60
AB  - To maintain an elevated glycolytic rate, cancerous or proliferating cells alter 
      the expression pattern of rate limiting glycolytic enzymes. Since glucose 
      phosphorylation is the first step in glycolysis, hexokinase (HK), the first rate 
      limiting glycolytic enzyme, can play a key regulatory role in this process. A 
      low-Km, mitochondrial type II-like tumor HK is described as the predominant form 
      in hepatomas. However, recent identification of a high-Km glucose phosphorylating 
      activity in a range of cancer cells prompted us to characterize glucose 
      phosphorylating enzymes of cancer cells at the molecular level. Highly sensitive 
      reverse-transcription polymerase chain reaction identifies an induction and 
      overexpression of a type II-like tumor HK RNA in a range of cancer cell lines 
      irrespective of tissue origin. In addition, we report here the identification of 
      two RNA transcripts of type II-like tumor HK of approximately 5.5 and 
      approximately 4.0 kb in these cancer cells lines, including muscle-derived L6 
      myoblast cells. Interestingly, under normal conditions muscle cells express only 
      a approximately 5.5-kb type II HK RNA transcript. A significant amount of type I 
      HK RNA was also found expressed in cancer cell lines. RNA encoding glucokinase 
      (GK), the high-Km HK isozyme, was found only in cancer cells originating from 
      liver and pancreas, which express GK under normal conditions.
FAU - Sebastian, S
AU  - Sebastian S
AD  - National Center for Cell Science, University of Pune Campus, India.
FAU - Kenkare, U W
AU  - Kenkare UW
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (Isoenzymes)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Messenger)
RN  - 56-73-5 (Glucose-6-Phosphate)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.2 (Glucokinase)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Glucokinase/metabolism
MH  - Glucose-6-Phosphate/metabolism
MH  - Glycolysis
MH  - Hexokinase/*metabolism
MH  - Isoenzymes/*metabolism
MH  - Neoplasm Proteins/*metabolism
MH  - Neoplasms/*metabolism
MH  - Polymerase Chain Reaction
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Tumor Cells, Cultured/enzymology
EDAT- 1998/07/29 02:04
MHDA- 2000/10/06 11:01
CRDT- 1998/07/29 02:04
PHST- 1998/07/29 02:04 [pubmed]
PHST- 2000/10/06 11:01 [medline]
PHST- 1998/07/29 02:04 [entrez]
AID - tbi19253 [pii]
AID - 10.1159/000030015 [doi]
PST - ppublish
SO  - Tumour Biol. 1998;19(4):253-60. doi: 10.1159/000030015.