PMID- 9679924
OWN - NLM
STAT- MEDLINE
DCOM- 19980813
LR  - 20061115
IS  - 0022-5347 (Print)
IS  - 0022-5347 (Linking)
VI  - 160
IP  - 2
DP  - 1998 Aug
TI  - Detection of deletions in the short arm of chromosome 3 in uncultured renal cell 
      carcinomas by interphase cytogenetics.
PG  - 534-9
AB  - PURPOSE: Analysis of genetic alterations may facilitate the differential 
      diagnosis of renal cell carcinoma (RCC) subtypes. For genetic classification, 
      deletion of the short arm of chromosome 3 (3p), the hallmark of 
      nonpapillary/clear cell RCC, is a major diagnostic criterion. Because of the 
      limited routine applicability of cytogenetics and molecular genetic techniques we 
      investigated interphase fluorescence in situ hybridization (FISH) for the 
      detection of this aberration in RCC. MATERIALS AND METHODS: Using seven 
      chromosome 3 specific probes FISH was performed on isolated nuclei from 26 
      uncultured sporadic RCC. RESULTS: Alterations of chromosome 3 were identified in 
      19 RCC (73%). Monosomy and/or 3p-deletions were observed in 15 of 19 (79%) 
      non-papillary/clear cell RCC but not in other morphologic subgroups. The median 
      percentage of cells in a specimen containing loss of 3p was 45%. Deletion mapping 
      indicated that large deletions affecting different regions in 3p are predominant. 
      Chromosomal region 3p24 was recurrently involved in all RCC with a deletion in 
      3p. CONCLUSION: Interphase FISH for the detection of loss in 3p provides a 
      sensitive and feasible method for the genetic classification of kidney tumors and 
      the delineation of recurrently deleted regions in 3p.
FAU - Siebert, R
AU  - Siebert R
AD  - Department of Human Genetics, University of Kiel, Germany.
FAU - Jacobi, C
AU  - Jacobi C
FAU - Matthiesen, P
AU  - Matthiesen P
FAU - Zuhlke-Jenisch, R
AU  - Zuhlke-Jenisch R
FAU - Potratz, C
AU  - Potratz C
FAU - Zhang, Y
AU  - Zhang Y
FAU - Stockle, M
AU  - Stockle M
FAU - Kloppel, G
AU  - Kloppel G
FAU - Grote, W
AU  - Grote W
FAU - Schlegelberger, B
AU  - Schlegelberger B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Urol
JT  - The Journal of urology
JID - 0376374
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Renal Cell/classification/diagnosis/*genetics
MH  - Chromosome Aberrations/genetics
MH  - *Chromosome Deletion
MH  - Chromosomes, Human, Pair 3/*genetics
MH  - Cytogenetics
MH  - Diagnosis, Differential
MH  - Feasibility Studies
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Kidney Neoplasms/classification/diagnosis/*genetics
MH  - Loss of Heterozygosity/genetics
MH  - Male
MH  - Middle Aged
MH  - Molecular Biology
MH  - Monosomy/genetics
MH  - Sensitivity and Specificity
EDAT- 1998/07/29 00:00
MHDA- 1998/07/29 00:01
CRDT- 1998/07/29 00:00
PHST- 1998/07/29 00:00 [pubmed]
PHST- 1998/07/29 00:01 [medline]
PHST- 1998/07/29 00:00 [entrez]
AID - S0022-5347(01)62954-9 [pii]
PST - ppublish
SO  - J Urol. 1998 Aug;160(2):534-9.