PMID- 9680693
OWN - NLM
STAT- MEDLINE
DCOM- 19980817
LR  - 20190729
IS  - 1060-3271 (Print)
IS  - 1060-3271 (Linking)
VI  - 81
IP  - 4
DP  - 1998 Jul-Aug
TI  - Liquid chromatographic determination of oxytetracycline in edible fish fillets 
      from six species of fish.
PG  - 702-8
AB  - The approved use of oxytetracycline (OTC) in U.S. aquaculture is limited to 
      specific diseases in salmonids and channel catfish. OTC may also be effective in 
      controlling diseases in other fish species important to public aquaculture, but 
      before approved use of OTC can be augmented, an analytical method for determining 
      OTC in fillet tissue from multiple species of fish will be required to support 
      residue depletion studies. The objective of this study was to develop and 
      validate a liquid chromatographic (LC) method that is accurate, precise, and 
      sensitive for OTC in edible fillets from multiple species of fish. Homogenized 
      fillet tissues from walleye, Atlantic salmon, striped bass, white sturgeon, 
      rainbow trout, and channel catfish were fortified with OTC at nominal 
      concentrations of 10, 20, 100, 1000, and 5000 ng/g. In tissues fortified with OTC 
      at 100, 1000, and 5000 ng/g, mean recoveries ranged from 83 to 90%, and relative 
      standard deviations (RSDs) ranged from 0.9 to 5.8%. In all other tissues, mean 
      recoveries ranged from 59 to 98%, and RSDs ranged from 3.3 to 20%. Method 
      quantitation limits ranged from 6 to 22 ng/g for the 6 species. The LC parameters 
      produced easily integratable OTC peaks without coelution of endogenous compounds. 
      The method is accurate, precise, and sensitive for OTC in fillet tissue from 6 
      species of fish from 5 phylogenetically diverse groups.
FAU - Meinertz, J R
AU  - Meinertz JR
AD  - U.S. Geological Survey, Biological Resources Division, Upper Mississippi Science 
      Center, La Crosse, WI 54602-0818, USA.
FAU - Stehly, G R
AU  - Stehly GR
FAU - Gingerich, W H
AU  - Gingerich WH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - J AOAC Int
JT  - Journal of AOAC International
JID - 9215446
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Solutions)
RN  - X20I9EN955 (Oxytetracycline)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis
MH  - Chromatography, Liquid
MH  - Fishes/*metabolism
MH  - Indicators and Reagents
MH  - Meat/*analysis
MH  - Oxytetracycline/*analysis
MH  - Solutions
EDAT- 1998/07/29 00:00
MHDA- 1998/07/29 00:01
CRDT- 1998/07/29 00:00
PHST- 1998/07/29 00:00 [pubmed]
PHST- 1998/07/29 00:01 [medline]
PHST- 1998/07/29 00:00 [entrez]
PST - ppublish
SO  - J AOAC Int. 1998 Jul-Aug;81(4):702-8.