PMID- 9680996
OWN - NLM
STAT- MEDLINE
DCOM- 19980818
LR  - 20221207
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 13
IP  - 4
DP  - 1998 Feb
TI  - FISH studies reveal the molecular and chromosomal organization of individual 
      telomere domains in tomato.
PG  - 507-17
AB  - The molecular and cytological organization of the telomeric repeat (TR) and the 
      subtelomeric repeat (TGR1) of tomato were investigated by fluorescence in situ 
      hybridization (FISH) techniques. Hybridization signals on extended DNA fibres, 
      visualized as linear fluorescent arrays representing individual telomeres, 
      unequivocally demonstrated the molecular co-linear arrangement of both repeats. 
      The majority of the telomeres consisted of a TR and a TGR1 region separated by a 
      spacer. Microscopic measurements of the TR and TGR1 signals revealed high 
      variation in length of both repeats, with maximum sizes of 223 and 1330 kb, 
      respectively. A total of 27 different combinations of TR and TGR1 was detected, 
      suggesting that all chromosome ends have their own unique telomere organization. 
      The fluorescent tracks on the extended DNA fibres were subdivided into four 
      classes: (i) TR-spacer-TGR1; (ii) TR-TGR1; (iii) only TR; (iv) only TGR1. FISH to 
      pachytene chromosomes enabled some of the TR/TGR1 groups to be assigned to 
      specific chromosome ends and to interstitial regions. These signals also provided 
      evidence for a reversed order of the TR and TGR1 sites at the native chromosome 
      ends, suggesting a backfolding telomere structure with the TGR1 repeats occupying 
      the most terminal position of the chromosomes. The FISH signals on diakinesis 
      chromosomes revealed that distal euchromatin areas and flanking telomeric 
      heterochromatin remained highly decondensed around the chiasmata in the 
      euchromatic chromosome areas. The rationale for the occurrence and distribution 
      of the TR and TGR1 repeats on the tomato chromosomes are discussed.
FAU - Zhong, X B
AU  - Zhong XB
AD  - Department of Molecular Biology, Wageningen Agricultural University, The 
      Netherlands.
FAU - Fransz, P F
AU  - Fransz PF
FAU - Wennekes-Eden, J
AU  - Wennekes-Eden J
FAU - Ramanna, M S
AU  - Ramanna MS
FAU - van Kammen, A
AU  - van Kammen A
FAU - Zabel, P
AU  - Zabel P
FAU - Hans de Jong, J
AU  - Hans de Jong J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (DNA, Plant)
SB  - IM
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Chromosomes/genetics/ultrastructure
MH  - DNA, Plant/genetics
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - In Situ Hybridization, Fluorescence
MH  - Solanum lycopersicum/*genetics/ultrastructure
MH  - Repetitive Sequences, Nucleic Acid
MH  - Telomere/*genetics
EDAT- 1998/07/29 00:00
MHDA- 1998/07/29 00:01
CRDT- 1998/07/29 00:00
PHST- 1998/07/29 00:00 [pubmed]
PHST- 1998/07/29 00:01 [medline]
PHST- 1998/07/29 00:00 [entrez]
AID - 10.1046/j.1365-313x.1998.00055.x [doi]
PST - ppublish
SO  - Plant J. 1998 Feb;13(4):507-17. doi: 10.1046/j.1365-313x.1998.00055.x.