PMID- 9681830
OWN - NLM
STAT- MEDLINE
DCOM- 19980804
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 16
IP  - 25
DP  - 1998 Jun 25
TI  - Nucleocytoplasmic transport of HTLV-1 RNA is regulated by two independent LTR 
      encoded nuclear retention elements.
PG  - 3309-16
AB  - Appropriate expression of HTLV-1 genes requires transcriptional transactivation 
      by Tax and post-transcriptional regulation by Rex, both mediated by LTR encoded 
      RNA sequences. Using a combination of deletion mutagenesis, Rex-reporter CAT 
      assays, fluorescence in situ hybridization (FISH) and confocal laser scanning 
      microscopy it was established that in the absence of Rex, CAT mRNAs harboring 
      HTLV-1 LTR sequences were unable to leave the nucleus. Deletion of the known U5 
      encoded cis-acting repressing sequence (CRS) led to a partial release of nuclear 
      retention. A novel regulatory element overlapping the 3' Rex responsive element 
      (RxRE) region was shown to prevent export and expression of these transcripts. 
      Deletion of both the 5' LTR encoded CRS and 3' LTR encoded downstream repressive 
      sequence (3' CRS) led to constitutive mRNA nuclear export and gene expression, 
      independently of Rex. The locations of the two regulatory elements indicate that 
      while the 5' CRS selectively acts to hinder export of unspliced transcripts, the 
      3' CRS has the capacity to induce nuclear retention of all HTLV-1 transcripts, 
      and therefore could potentially contribute to viral latency in infected cells.
FAU - King, J A
AU  - King JA
AD  - Department of Cellular Immunology, German Cancer Research Center, Heidelberg.
FAU - Bridger, J M
AU  - Bridger JM
FAU - Lochelt, M
AU  - Lochelt M
FAU - Lichter, P
AU  - Lichter P
FAU - Schulz, T F
AU  - Schulz TF
FAU - Schirrmacher, V
AU  - Schirrmacher V
FAU - Khazaie, K
AU  - Khazaie K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Repressor Proteins)
RN  - 0 (Ribonucleoprotein, U5 Small Nuclear)
SB  - IM
MH  - Animals
MH  - Biological Transport/genetics/physiology
MH  - COS Cells
MH  - Cell Nucleus/chemistry/*metabolism/virology
MH  - Cells, Cultured
MH  - Cytoplasm/chemistry/*metabolism/virology
MH  - Cytoplasmic Streaming/genetics
MH  - Gene Expression Regulation, Viral
MH  - HeLa Cells
MH  - Human T-lymphotropic virus 1/chemistry/*genetics/*metabolism
MH  - Humans
MH  - Jurkat Cells
MH  - RNA Processing, Post-Transcriptional/genetics/physiology
MH  - RNA, Messenger/metabolism
MH  - RNA, Viral/*metabolism
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Repressor Proteins/genetics/physiology
MH  - Ribonucleoprotein, U5 Small Nuclear/genetics/*physiology
MH  - Transcription, Genetic/genetics/physiology
EDAT- 1998/07/29 00:00
MHDA- 1998/07/29 00:01
CRDT- 1998/07/29 00:00
PHST- 1998/07/29 00:00 [pubmed]
PHST- 1998/07/29 00:01 [medline]
PHST- 1998/07/29 00:00 [entrez]
AID - 10.1038/sj.onc.1201884 [doi]
PST - ppublish
SO  - Oncogene. 1998 Jun 25;16(25):3309-16. doi: 10.1038/sj.onc.1201884.