PMID- 9681841
OWN - NLM
STAT- MEDLINE
DCOM- 19980813
LR  - 20190905
IS  - 0954-6820 (Print)
IS  - 0954-6820 (Linking)
VI  - 243
IP  - 6
DP  - 1998 Jun
TI  - The search for the MEN1 gene. The European Consortium on MEN-1.
PG  - 441-6
AB  - The search for the gene whose mutations predispose individuals to multiple 
      endocrine neoplasia type 1 (MEN-1) started in 1988 when the MEN1 locus was 
      assigned to 11q13, close to PYGM. It came to an end with the recent 
      identification of a gene expressed ubiquitously which harbours inactivating 
      mutations associated with MEN-1. During these nine years, the genetic linkage 
      interval had been slowly reduced, and losses of heterozygosity (LOH) in MEN-1 
      tumours had given strong indications that MEN1 was a tumour suppressor gene. It 
      is ironic that MEN1 was finally found to be located less than 100 kb telomeric to 
      PYGM. From the beginning, this gene was the most tightly linked genetically to 
      MEN-1. In addition, LOH had already shown (in 1990) that it was the most likely 
      centromeric boundary of the MEN1 minimal region. We recently narrowed the 
      critical region to 900 kb through meiotic mapping, and established a 1200-kb 
      sequence-ready contig consisting of cosmids, bacterial artificial chromosomes 
      (BACs) and P1-derived artificial chromosomes (PACs), including three gene 
      clusters (19 genes and 3 expressed sequence tags). Taking LOH results into 
      account, the gene was likely to be present in the 300-kb area telomeric to PYGM 
      that we had covered with BACs. One of the novel genes that we have identified by 
      cDNA selection in this region, SCG2 (Suppressor Candidate Gene 2), proved to be 
      identical to the recently published MEN1 gene. Mutation analysis of SCG2 in 11 
      unrelated MEN-1 families identified one nucleotide sequence polymorphism and 10 
      different mutations that segregated with the disease.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - J Intern Med
JT  - Journal of internal medicine
JID - 8904841
SB  - IM
MH  - Cloning, Molecular
MH  - Europe
MH  - Genes, Tumor Suppressor/genetics
MH  - Humans
MH  - Multiple Endocrine Neoplasia Type 1/*genetics
MH  - *Mutation
RF  - 33
EDAT- 1998/07/29 00:00
MHDA- 1998/07/29 00:01
CRDT- 1998/07/29 00:00
PHST- 1998/07/29 00:00 [pubmed]
PHST- 1998/07/29 00:01 [medline]
PHST- 1998/07/29 00:00 [entrez]
AID - 10.1046/j.1365-2796.1998.00347.x [doi]
PST - ppublish
SO  - J Intern Med. 1998 Jun;243(6):441-6. doi: 10.1046/j.1365-2796.1998.00347.x.