PMID- 9681880
OWN - NLM
STAT- MEDLINE
DCOM- 19981001
LR  - 20190831
IS  - 0047-6374 (Print)
IS  - 0047-6374 (Linking)
VI  - 103
IP  - 1
DP  - 1998 Jun 1
TI  - Binding of circulating antibodies to reactive oxygen species modified-DNA and 
      detecting DNA damage by a monoclonal antibody probe.
PG  - 69-80
AB  - Circulating antibodies in the sera of normal, healthy humans of different age 
      groups to native DNA (nDNA) and reactive oxygen species modified DNA (ROS-DNA) 
      was studied by competition ELISA. Sera from the young population (< 50 years of 
      age) showed negligible levels of anti-DNA antibodies. In contrast, anti-DNA 
      antibodies were found in three sera from the moderately aged group (50-59 years) 
      with a high binding to ROS-DNA (43-47%). In the aged group (> 60 years), four 
      sera showed higher recognition of ROS-DNA (> 60% inhibition) over nDNA (55-60%). 
      Oxidative lesions in human genomic DNA were immunochemically detected using the 
      monoclonal anti-ROS-DNA antibody as a probe. The antibody has a high specificity 
      for ROS-DNA and preferentially recognizes ROS-modified epitopes on nucleic acids. 
      The study indicates low recognition of DNA isolated from the younger population, 
      while in the age group 50-59 years, one DNA isolate showed a high inhibition 
      (57%) in monoclonal antibody binding. Four DNA isolates from the aged group 
      showed substantial inhibition in antibody activity to the extent of 49, 53, 64 
      and 69%. The results demonstrate an age-related increase in the levels of 
      anti-DNA antibodies, with a higher recognition and binding to ROS-DNA. A high 
      reactivity of DNA isolated from aged individuals by the monoclonal antibody 
      indicates increased oxidative stress leading to DNA damage. It is suggested that 
      free radical damage to DNA in vivo, particularly in the aged, alters its 
      antigenicity and stimulates an immune response against modified DNA. These 
      antibodies are cross-reactive to nDNA.
FAU - Ashok, B T
AU  - Ashok BT
AD  - Department of Biochemistry, Faculty of Medicine, Aligarh Muslim University, 
      India.
FAU - Ali, R
AU  - Ali R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Mech Ageing Dev
JT  - Mechanisms of ageing and development
JID - 0347227
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Reactive Oxygen Species)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aging/*immunology/metabolism
MH  - Antibodies, Antinuclear/*blood
MH  - *Antibodies, Monoclonal
MH  - Antibody Specificity
MH  - DNA/blood/*chemistry/*immunology
MH  - DNA Damage/*immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Middle Aged
MH  - Reactive Oxygen Species/metabolism
EDAT- 1998/07/29 00:00
MHDA- 1998/07/29 00:01
CRDT- 1998/07/29 00:00
PHST- 1998/07/29 00:00 [pubmed]
PHST- 1998/07/29 00:01 [medline]
PHST- 1998/07/29 00:00 [entrez]
AID - S0047-6374(98)00025-6 [pii]
AID - 10.1016/s0047-6374(98)00025-6 [doi]
PST - ppublish
SO  - Mech Ageing Dev. 1998 Jun 1;103(1):69-80. doi: 10.1016/s0047-6374(98)00025-6.