PMID- 9684045
OWN - NLM
STAT- MEDLINE
DCOM- 19981016
LR  - 20181113
IS  - 0830-9000 (Print)
IS  - 0830-9000 (Linking)
VI  - 62
IP  - 3
DP  - 1998 Jul
TI  - Genomic and antigenic variations of porcine reproductive and respiratory syndrome 
      virus major envelope GP5 glycoprotein.
PG  - 170-7
AB  - The objective of the present study was to evaluate the importance of genomic and 
      antigenic variations which may have affected the major envelope glycoprotein GP5 
      of porcine reproductive and respiratory syndrome virus (PRRSV) isolates 
      responsible for outbreaks in Quebec and Ontario, in comparison with the 
      modified-live U.S. vaccine strain (MLV) and the European prototype strain from 
      Lelystad (LV). Nucleotide sequence analyses of the open reading frame (ORF)5 
      genes showed that all of the isolates studied were heterogenous, amino acid (aa) 
      identities varied from 88 to 99% with the MLV strain, and between 51 and 54% with 
      the LV strain. The aa substitutions were randomly scattered across the protein, 
      although one region between residues 26 and 39 was found to correspond to a 
      hypervariable region which involved 0 to 3 potential N-glycosylation sites. The 
      ORF5 encoded products of 5 of these isolates, including the MLV and LV strains, 
      were expressed in E. coli as recombinant proteins fused to the glutathione 
      S-transferase (GST) protein and used to raise hyperimmune anti-ORF5 sera in 
      rabbits. The reactivity patterns of strain-specific hyperimmune anti-ORF5 sera 
      and a panel of 4 monoclonal antibodies directed against the ORF5 gene product of 
      the Quebec IAF-Klop strain of PRRSV, indicated that GP5 of field isolates also 
      underwent antigenic variations. The data suggest that neutralizing epitopes, 
      independent of conformation and glycosylation, are also associated with antigenic 
      variability of the GP5 of PRRSV.
FAU - Pirzadeh, B
AU  - Pirzadeh B
AD  - Centre de recherche en virologie, Institut Armand-Frappier, Universite du Quebec.
FAU - Gagnon, C A
AU  - Gagnon CA
FAU - Dea, S
AU  - Dea S
LA  - eng
SI  - GENBANK/AF013106
SI  - GENBANK/U64928
SI  - GENBANK/U64929
SI  - GENBANK/U64930
SI  - GENBANK/U64931
SI  - GENBANK/U64932
SI  - GENBANK/U64933
SI  - GENBANK/U64934
SI  - GENBANK/U64935
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Can J Vet Res
JT  - Canadian journal of veterinary research = Revue canadienne de recherche 
      veterinaire
JID - 8607793
RN  - 0 (DNA Primers)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - *Antigenic Variation
MH  - Base Sequence
MH  - DNA Primers
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Phylogeny
MH  - Point Mutation
MH  - Polymerase Chain Reaction
MH  - Porcine Reproductive and Respiratory Syndrome/virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/*genetics/immunology/isolation & purification
MH  - Rabbits
MH  - Sequence Alignment
MH  - Sequence Homology, Amino Acid
MH  - Species Specificity
MH  - Swine
MH  - Viral Envelope Proteins/chemistry/*genetics
PMC - PMC1189472
EDAT- 1998/07/31 00:00
MHDA- 1998/07/31 00:01
PMCR- 1998/10/01
CRDT- 1998/07/31 00:00
PHST- 1998/07/31 00:00 [pubmed]
PHST- 1998/07/31 00:01 [medline]
PHST- 1998/07/31 00:00 [entrez]
PHST- 1998/10/01 00:00 [pmc-release]
PST - ppublish
SO  - Can J Vet Res. 1998 Jul;62(3):170-7.