PMID- 9684732
OWN - NLM
STAT- MEDLINE
DCOM- 19981021
LR  - 20190727
IS  - 0049-3848 (Print)
IS  - 0049-3848 (Linking)
VI  - 90
IP  - 3
DP  - 1998 May 1
TI  - A stable, multi-subunit complex of beta2glycoprotein I.
PG  - 131-7
AB  - Beta2glycoprotein I (beta2GPI) is a 54-kDa plasma protein which is recognized as 
      an autoantigen for antibodies from patients with antiphospholipid syndrome (APS). 
      SDS-PAGE (under reducing conditions) of beta2GPI from three sources indicates 
      that the 54-kDa beta2GPI band is accompanied by a band corresponding to an 8-kDa 
      protein. In the absence of detergent and reducing agents (native PAGE), beta2GPI 
      demonstrated a large complex (molecular mass approximately 320 kDa) which is 
      dissociable by boiling in 6-8 M urea, yielding several lower molecular mass 
      bands, one of which corresponds to the 8-kDa protein observed in SDS-PAGE. Sera 
      from five healthy adults demonstrated native beta2GPI migration equivalent to the 
      commercially purified protein. Atomic force microscopy (AFM) images of native 
      beta2GPI show aggregrates of particles each having a diameter of 30-35 nm. This 
      is consistent with a globular unit the size of which would be substantially 
      larger than that expected for a 54-kDa protein. These experiments suggest that 
      the 54-kDa beta2GPI monomer subunits exist as a multimeric complex with the 8-kDa 
      protein.
FAU - Galazka, M
AU  - Galazka M
AD  - Department of Chemistry, College of Arts and Sciences, School of Graduate Medical 
      Education, Seton Hall University, South Orange, New Jersey, USA.
FAU - Keil, L B
AU  - Keil LB
FAU - Kohles, J D
AU  - Kohles JD
FAU - Li, J
AU  - Li J
FAU - Kelty, S P
AU  - Kelty SP
FAU - Petersheim, M
AU  - Petersheim M
FAU - DeBari, V A
AU  - DeBari VA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Thromb Res
JT  - Thrombosis research
JID - 0326377
RN  - 0 (Detergents)
RN  - 0 (Disulfides)
RN  - 0 (Glycoproteins)
RN  - 0 (Reducing Agents)
RN  - 0 (beta 2-Glycoprotein I)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
SB  - IM
MH  - Adult
MH  - Blotting, Western
MH  - Detergents/pharmacology
MH  - Disulfides/pharmacology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Glycoproteins/blood/*chemistry/drug effects
MH  - Humans
MH  - Microscopy, Atomic Force
MH  - Molecular Weight
MH  - Protein Conformation
MH  - Reducing Agents/pharmacology
MH  - Sodium Dodecyl Sulfate
MH  - beta 2-Glycoprotein I
EDAT- 1998/07/31 00:00
MHDA- 1998/07/31 00:01
CRDT- 1998/07/31 00:00
PHST- 1998/07/31 00:00 [pubmed]
PHST- 1998/07/31 00:01 [medline]
PHST- 1998/07/31 00:00 [entrez]
AID - S0049-3848(98)00047-4 [pii]
AID - 10.1016/s0049-3848(98)00047-4 [doi]
PST - ppublish
SO  - Thromb Res. 1998 May 1;90(3):131-7. doi: 10.1016/s0049-3848(98)00047-4.