PMID- 9685522
OWN - NLM
STAT- MEDLINE
DCOM- 19981020
LR  - 20190831
IS  - 0171-967X (Print)
IS  - 0171-967X (Linking)
VI  - 63
IP  - 2
DP  - 1998 Aug
TI  - A calcium release activated calcium influx in primary cultures of rat 
      osteoblast-like cells.
PG  - 154-9
AB  - Osteoblast-like (OBL) cells in primary culture were tested for their ability to 
      generate a calcium release activated calcium flux (CRAC). Influx of Ca2+ was 
      optically detected by fura-2. Intracellular calcium stores (ICS) were emptied in 
      the absence of extracellular calcium ([Ca2+]e) by 5 microM thapsigargin (TG) or 2 
      microM A23187. Readdition of 1.8 mM [Ca2+]e increased the free intracellular Ca2+ 
      ([Ca2+]i) after a delay of 30-60 seconds at a rate of 2.3 nM/s due to CRAC. This 
      rate depended on [Ca2+]e and was substantially lowered if readdition of 1.8 mM 
      [Ca2+]e was preceded by, e.g., 0.72 mM [Ca2+]e. CRAC-induced [Ca2+]i peaks were 
      correlated (r = 0.543) with [Ca2+]i peaks during the complete depletion of ICS 
      with A23187. Ca2+ influx due to CRAC could be blocked by flufenamic acid (100 
      microM) but not verapamil (20 microM). Ni2+ (1 mM), although reversibly blocking 
      CRAC, accelerated the initial [Ca2+]i influx rate. Induction of CRAC enhanced the 
      influx of Mn2+ 4.3-fold, as measured by quenching of fura-2 fluorescence. In 
      summary, OBL cells exhibit a CRAC which allows for the permeation of ions other 
      than Ca2+. This Ca2+ flux may be activated by transmembraneous gradients of Ca2+ 
      and Ni2+.
FAU - Wiemann, M
AU  - Wiemann M
AD  - Institut fur Physiologie, Universitat-GH Essen, Germany.
FAU - Busselberg, D
AU  - Busselberg D
FAU - Schirrmacher, K
AU  - Schirrmacher K
FAU - Bingmann, D
AU  - Bingmann D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Calcif Tissue Int
JT  - Calcified tissue international
JID - 7905481
RN  - 0 (Calcium Channels)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 42Z2K6ZL8P (Manganese)
RN  - 60GCX7Y6BH (Flufenamic Acid)
RN  - 67526-95-8 (Thapsigargin)
RN  - 7OV03QG267 (Nickel)
RN  - CJ0O37KU29 (Verapamil)
RN  - SY7Q814VUP (Calcium)
RN  - TSN3DL106G (Fura-2)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Calcimycin/pharmacology
MH  - Calcium/*metabolism
MH  - Calcium Channels/physiology
MH  - Cells, Cultured
MH  - Flufenamic Acid/pharmacology
MH  - Fura-2
MH  - Manganese/metabolism
MH  - Nickel/metabolism/pharmacology
MH  - Osteoblasts/drug effects/*metabolism
MH  - Rats
MH  - Spectrometry, Fluorescence
MH  - Thapsigargin/pharmacology
MH  - Verapamil/pharmacology
EDAT- 1998/08/01 00:00
MHDA- 1998/08/01 00:01
CRDT- 1998/08/01 00:00
PHST- 1998/08/01 00:00 [pubmed]
PHST- 1998/08/01 00:01 [medline]
PHST- 1998/08/01 00:00 [entrez]
AID - 10.1007/s002239900507 [doi]
PST - ppublish
SO  - Calcif Tissue Int. 1998 Aug;63(2):154-9. doi: 10.1007/s002239900507.