PMID- 9686763
OWN - NLM
STAT- MEDLINE
DCOM- 19980811
LR  - 20190706
IS  - 0009-7330 (Print)
IS  - 0009-7330 (Linking)
VI  - 83
IP  - 2
DP  - 1998 Jul 27
TI  - Increased expression of estrogen receptor-beta mRNA in male blood vessels after 
      vascular injury.
PG  - 224-9
AB  - Estrogen exerts direct effects on vascular endothelial and smooth muscle cells 
      that are important for vascular protection. Estrogen receptor-alpha (ERalpha) is 
      expressed in vascular cells from males and females and may mediate some of the 
      effects of estrogen on vascular tissue. However, we recently found that estrogen 
      is able to protect against vascular injury in ovariectomized female ERalpha 
      knockout mice. These mice express the newly described estrogen receptor-beta 
      (ERbeta) in their aortas, suggesting that ERbeta may also mediate some of the 
      direct effects of estrogen on the vasculature. In this study, the level of 
      expression of ERalpha and ERbeta mRNA in male rat aortas was examined before and 
      after vascular injury using en face (Hautchen) preparations and in situ 
      hybridization. Little or no change in ERalpha expression was observed after 
      vascular injury in either vascular endothelial or smooth muscle cells at any time 
      point. In contrast, ERbeta mRNA was found to be expressed markedly after balloon 
      injury. In endothelial cells, ERbeta was increased by 2 days after injury, and 
      high levels of expression were maintained at 8 and 14 days. Furthermore, ERbeta 
      expression was high in luminal smooth muscle cells at 8 and 14 days after injury 
      and had decreased to low levels by 28 days after injury. These data demonstrate 
      the presence of ERbeta in male vascular tissues and the induction of ERbeta mRNA 
      expression after vascular injury, supporting a role for ERbeta in the direct 
      vascular effects of estrogen.
FAU - Lindner, V
AU  - Lindner V
AD  - Maine Medical Center, Portland, USA.
FAU - Kim, S K
AU  - Kim SK
FAU - Karas, R H
AU  - Karas RH
FAU - Kuiper, G G
AU  - Kuiper GG
FAU - Gustafsson, J A
AU  - Gustafsson JA
FAU - Mendelsohn, M E
AU  - Mendelsohn ME
LA  - eng
GR  - HL-30386/HL/NHLBI NIH HHS/United States
GR  - HL-56069/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Circ Res
JT  - Circulation research
JID - 0047103
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Estrogen)
SB  - IM
MH  - Animals
MH  - Aorta/*injuries/pathology
MH  - Catheterization/adverse effects
MH  - Estrogen Receptor alpha
MH  - Estrogen Receptor beta
MH  - Gene Expression Regulation
MH  - In Situ Hybridization
MH  - Male
MH  - RNA, Messenger/*biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Estrogen/*biosynthesis/genetics
MH  - Sex Characteristics
MH  - Tunica Intima/*metabolism
EDAT- 1998/08/01 00:00
MHDA- 1998/08/01 00:01
CRDT- 1998/08/01 00:00
PHST- 1998/08/01 00:00 [pubmed]
PHST- 1998/08/01 00:01 [medline]
PHST- 1998/08/01 00:00 [entrez]
AID - 10.1161/01.res.83.2.224 [doi]
PST - ppublish
SO  - Circ Res. 1998 Jul 27;83(2):224-9. doi: 10.1161/01.res.83.2.224.