PMID- 9688521
OWN - NLM
STAT- MEDLINE
DCOM- 19980923
LR  - 20191102
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 6
IP  - 4
DP  - 1998 Jun
TI  - Identification of 16 chicken microchromosomes by molecular markers using 
      two-colour fluorescence in situ hybridization (FISH).
PG  - 307-13
AB  - A feature of avian karyotypes is the presence of microchromosomes. As a typical 
      avian genome, the chicken karyotype (2n = 78) consists of nine pairs of 
      macrochromosomes, including the W and Z sexual chromosomes, and 30 pairs of 
      indistinguishable microchromosomes usually ordered arbitrarily by decreasing 
      size. Despite their reduced size, microchromosomes represent one-third of the 
      genome and have a high gene density. So as to provide a tool to identify them, we 
      developed a set of large insert-containing clones to be used as tags in 
      two-colour fluorescence in situ hybridization experiments. Seventeen clones, six 
      of which contain a microsatellite sequence and two others the fatty acid synthase 
      gene or genes from the major histocompatibility complex, all presenting a strong 
      hybridization signal, were selected for this purpose and enabled us to identify 
      16 different microchromosomes. The ability to recognize individual 
      microchromosomes will be of great value for cytogenetic gene mapping, assignation 
      of linkage groups from genetic maps and other studies on avian genome structure.
FAU - Fillon, V
AU  - Fillon V
AD  - Laboratoire de Genetique Cellulaire, Centre INRA de Toulouse-Auzeville, Castanet 
      Tolosan, France.
FAU - Morisson, M
AU  - Morisson M
FAU - Zoorob, R
AU  - Zoorob R
FAU - Auffray, C
AU  - Auffray C
FAU - Douaire, M
AU  - Douaire M
FAU - Gellin, J
AU  - Gellin J
FAU - Vignal, A
AU  - Vignal A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Genetic Markers)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
RN  - EC 2.3.1.85 (Fatty Acid Synthases)
SB  - IM
MH  - Animals
MH  - Chickens/*genetics
MH  - Chromosome Mapping/*methods
MH  - Chromosomes/classification/*genetics
MH  - Cloning, Molecular
MH  - DNA Probes
MH  - Fatty Acid Synthases/genetics
MH  - Fluorescent Dyes
MH  - Genes, MHC Class II
MH  - *Genetic Markers
MH  - *In Situ Hybridization, Fluorescence
MH  - Indoles
MH  - Karyotyping/methods
MH  - Microsatellite Repeats
EDAT- 1998/08/04 00:00
MHDA- 1998/08/04 00:01
CRDT- 1998/08/04 00:00
PHST- 1998/08/04 00:00 [pubmed]
PHST- 1998/08/04 00:01 [medline]
PHST- 1998/08/04 00:00 [entrez]
AID - 10.1023/a:1009274925051 [doi]
PST - ppublish
SO  - Chromosome Res. 1998 Jun;6(4):307-13. doi: 10.1023/a:1009274925051.