PMID- 9688522 OWN - NLM STAT- MEDLINE DCOM- 19980923 LR - 20191102 IS - 0967-3849 (Print) IS - 0967-3849 (Linking) VI - 6 IP - 4 DP - 1998 Jun TI - Terminal heterochromatin and alternative telomeric sequences in Allium cepa. PG - 315-21 AB - The chromosome termini of the onion (Allium cepa) apparently lack Arabidopsis-type telomeric repeats. The terminal Giemsa bands of A. cepa chromosomes contain a 375-bp satellite and (short arm of chromosome 8)/ or (short arm of chromosome 6) rDNA repeats. By means of fluorescence in situ hybridization (FISH) on metaphase chromosomes and on DNA fibres with probes specific for Ty1-copia retroelements and a En/Spm-transposable element-like sequence, respectively, it was demonstrated that the former are rarely and the latter frequently associated with satellite repeats within the terminal heterochromatin. Polymerase chain reaction of polyC-tailed nuclear DNA with anchor primers and single satellite-specific primers yielded amplification products that, after cloning and sequencing, revealed satellite sequences. This supports the idea that satellite repeats represent one class of terminal sequences in A. cepa. FAU - Pich, U AU - Pich U AD - Institut fur Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany. FAU - Schubert, I AU - Schubert I LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Netherlands TA - Chromosome Res JT - Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology JID - 9313452 RN - 0 (DNA Probes) RN - 0 (Heterochromatin) RN - 0 (Retroelements) SB - IM MH - Allium/*genetics MH - Base Sequence MH - Blotting, Southern MH - DNA Probes MH - Genome, Plant MH - Heterochromatin/*genetics MH - In Situ Hybridization, Fluorescence MH - Molecular Sequence Data MH - Polymerase Chain Reaction MH - Repetitive Sequences, Nucleic Acid MH - Retroelements MH - Telomere/*genetics EDAT- 1998/08/04 00:00 MHDA- 1998/08/04 00:01 CRDT- 1998/08/04 00:00 PHST- 1998/08/04 00:00 [pubmed] PHST- 1998/08/04 00:01 [medline] PHST- 1998/08/04 00:00 [entrez] AID - 10.1023/a:1009227009121 [doi] PST - ppublish SO - Chromosome Res. 1998 Jun;6(4):315-21. doi: 10.1023/a:1009227009121.