PMID- 9688588
OWN - NLM
STAT- MEDLINE
DCOM- 19980916
LR  - 20190322
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 275
IP  - 2
DP  - 1998 Aug
TI  - L-Arginine inhibits vasopressin-stimulated mesangial cell Ca2+.
PG  - C352-7
LID - 10.1152/ajpcell.1998.275.2.C352 [doi]
AB  - L-Arginine (L-Arg) affects various parameters that modulate the progression of 
      renal disease. These same factors [e.g., glomerular filtration rate, changes in 
      mesangial cell (MC) tension, and production of NO] are all controlled at least in 
      part by changes in MC intracellular Ca2+ concentration ([Ca2+]i). We therefore 
      evaluated the effect of L-Arg on MC [Ca2+]i. We found that L-Arg inhibits the 
      vasopressin-stimulated rise in MC [Ca2+]i both in rat and murine cell cultures. 
      This effect does not appear to be due to metabolism of L-Arg to either NO or 
      L-ornithine (L-Orn). Blocking the metabolism of L-Arg with 
      Nomega-monomethyl-L-arginine, an NO synthase inhibitor, or with 20 mM L-valine 
      (L-Val), an inhibitor of Orn formation, does not reverse the inhibition. However, 
      other cationic amino acids, as well guanidine, the functional group of L-Arg, all 
      inhibit the vasopressin-stimulated rise in [Ca2+]i, consistent with a structural 
      basis for this effect. We conclude that 1) L-Arg inhibits vasopressin-stimulated 
      murine and rat MC [Ca2+]i rise, 2) this inhibition is not mediated by metabolism 
      of L-Arg to either NO or L-Orn, and 3) the effect of L-Arg is due to its cationic 
      functional group, guanidine.
FAU - Balsam, L
AU  - Balsam L
AD  - Division of Nephrology, Department of Medicine, Nassau County Medical Center, 
      East Meadow, NY 11554, USA.
FAU - Nikbakht, N
AU  - Nikbakht N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 113-79-1 (Arginine Vasopressin)
RN  - 27JT06E6GR (omega-N-Methylarginine)
RN  - 2FZ7Y3VOQX (Spermine)
RN  - 94ZLA3W45F (Arginine)
RN  - JU58VJ6Y3B (Guanidine)
RN  - SY7Q814VUP (Calcium)
RN  - U87FK77H25 (Spermidine)
SB  - IM
MH  - Animals
MH  - Arginine/*pharmacology
MH  - Arginine Vasopressin/*pharmacology/physiology
MH  - Calcium/*metabolism
MH  - Cell Line, Transformed
MH  - Cells, Cultured
MH  - Glomerular Mesangium/cytology/drug effects/*physiology
MH  - Guanidine/pharmacology
MH  - Mice
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spermidine/pharmacology
MH  - Spermine/pharmacology
MH  - omega-N-Methylarginine/pharmacology
EDAT- 1998/08/04 00:00
MHDA- 1998/08/04 00:01
CRDT- 1998/08/04 00:00
PHST- 1998/08/04 00:00 [pubmed]
PHST- 1998/08/04 00:01 [medline]
PHST- 1998/08/04 00:00 [entrez]
AID - 10.1152/ajpcell.1998.275.2.C352 [doi]
PST - ppublish
SO  - Am J Physiol. 1998 Aug;275(2):C352-7. doi: 10.1152/ajpcell.1998.275.2.C352.